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X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

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X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples. / Schmutzler, Oliver; Graf, Sebastian; Behm, Nils; Mansour, Wael Y.; Blumendorf, Florian; Staufer, Theresa; Körnig, Christian; Salah, Dina; Kang, Yanan; Peters, Jan N.; Liu, Yang; Feliu, Neus; Parak, Wolfgang J.; Burkhardt, Anja; Gargioni, Elisabetta; Gennis, Sabrina; Chandralingam, Sharah; Höeg, Finn; Maison, Wolfgang; Rothkamm, Kai; Schulz, Florian; Grüner, Florian.

In: INT J MOL SCI, Vol. 22, No. 7, 01.04.2021, p. 3691.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schmutzler, O, Graf, S, Behm, N, Mansour, WY, Blumendorf, F, Staufer, T, Körnig, C, Salah, D, Kang, Y, Peters, JN, Liu, Y, Feliu, N, Parak, WJ, Burkhardt, A, Gargioni, E, Gennis, S, Chandralingam, S, Höeg, F, Maison, W, Rothkamm, K, Schulz, F & Grüner, F 2021, 'X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples', INT J MOL SCI, vol. 22, no. 7, pp. 3691. https://doi.org/10.3390/ijms22073691

APA

Schmutzler, O., Graf, S., Behm, N., Mansour, W. Y., Blumendorf, F., Staufer, T., Körnig, C., Salah, D., Kang, Y., Peters, J. N., Liu, Y., Feliu, N., Parak, W. J., Burkhardt, A., Gargioni, E., Gennis, S., Chandralingam, S., Höeg, F., Maison, W., ... Grüner, F. (2021). X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples. INT J MOL SCI, 22(7), 3691. https://doi.org/10.3390/ijms22073691

Vancouver

Schmutzler O, Graf S, Behm N, Mansour WY, Blumendorf F, Staufer T et al. X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples. INT J MOL SCI. 2021 Apr 1;22(7):3691. https://doi.org/10.3390/ijms22073691

Bibtex

@article{10997161e89844799a8c9ec1e1e8d094,
title = "X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples",
abstract = "Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.",
author = "Oliver Schmutzler and Sebastian Graf and Nils Behm and Mansour, {Wael Y.} and Florian Blumendorf and Theresa Staufer and Christian K{\"o}rnig and Dina Salah and Yanan Kang and Peters, {Jan N.} and Yang Liu and Neus Feliu and Parak, {Wolfgang J.} and Anja Burkhardt and Elisabetta Gargioni and Sabrina Gennis and Sharah Chandralingam and Finn H{\"o}eg and Wolfgang Maison and Kai Rothkamm and Florian Schulz and Florian Gr{\"u}ner",
note = "Bitte die o.g. Autoren (Nils Behm, Sabrina Gennis, Dina Salah) der UKE Strahlentherapie zuordnen.",
year = "2021",
month = apr,
day = "1",
doi = "10.3390/ijms22073691",
language = "English",
volume = "22",
pages = "3691",
journal = "INT J MOL SCI",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "7",

}

RIS

TY - JOUR

T1 - X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

AU - Schmutzler, Oliver

AU - Graf, Sebastian

AU - Behm, Nils

AU - Mansour, Wael Y.

AU - Blumendorf, Florian

AU - Staufer, Theresa

AU - Körnig, Christian

AU - Salah, Dina

AU - Kang, Yanan

AU - Peters, Jan N.

AU - Liu, Yang

AU - Feliu, Neus

AU - Parak, Wolfgang J.

AU - Burkhardt, Anja

AU - Gargioni, Elisabetta

AU - Gennis, Sabrina

AU - Chandralingam, Sharah

AU - Höeg, Finn

AU - Maison, Wolfgang

AU - Rothkamm, Kai

AU - Schulz, Florian

AU - Grüner, Florian

N1 - Bitte die o.g. Autoren (Nils Behm, Sabrina Gennis, Dina Salah) der UKE Strahlentherapie zuordnen.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

AB - Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

U2 - 10.3390/ijms22073691

DO - 10.3390/ijms22073691

M3 - SCORING: Journal article

VL - 22

SP - 3691

JO - INT J MOL SCI

JF - INT J MOL SCI

SN - 1661-6596

IS - 7

ER -