X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
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X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples. / Schmutzler, Oliver; Graf, Sebastian; Behm, Nils; Mansour, Wael Y.; Blumendorf, Florian; Staufer, Theresa; Körnig, Christian; Salah, Dina; Kang, Yanan; Peters, Jan N.; Liu, Yang; Feliu, Neus; Parak, Wolfgang J.; Burkhardt, Anja; Gargioni, Elisabetta; Gennis, Sabrina; Chandralingam, Sharah; Höeg, Finn; Maison, Wolfgang; Rothkamm, Kai; Schulz, Florian; Grüner, Florian.
in: INT J MOL SCI, Jahrgang 22, Nr. 7, 01.04.2021, S. 3691.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
AU - Schmutzler, Oliver
AU - Graf, Sebastian
AU - Behm, Nils
AU - Mansour, Wael Y.
AU - Blumendorf, Florian
AU - Staufer, Theresa
AU - Körnig, Christian
AU - Salah, Dina
AU - Kang, Yanan
AU - Peters, Jan N.
AU - Liu, Yang
AU - Feliu, Neus
AU - Parak, Wolfgang J.
AU - Burkhardt, Anja
AU - Gargioni, Elisabetta
AU - Gennis, Sabrina
AU - Chandralingam, Sharah
AU - Höeg, Finn
AU - Maison, Wolfgang
AU - Rothkamm, Kai
AU - Schulz, Florian
AU - Grüner, Florian
N1 - Bitte die o.g. Autoren (Nils Behm, Sabrina Gennis, Dina Salah) der UKE Strahlentherapie zuordnen.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
AB - Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
U2 - 10.3390/ijms22073691
DO - 10.3390/ijms22073691
M3 - SCORING: Journal article
VL - 22
SP - 3691
JO - INT J MOL SCI
JF - INT J MOL SCI
SN - 1661-6596
IS - 7
ER -