The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)
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The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). / Moquet, Jayne; Barnard, Stephen; Staynova, Albena; Lindholm, Carita; Monteiro Gil, Octávia; Martins, Vanda; Rößler, Ute; Vral, Anne; Vandevoorde, Charlot; Wojewódzka, Maria; Rothkamm, Kai.
In: INT J RADIAT BIOL, Vol. 93, No. 1, 01.2017, p. 58-64.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)
AU - Moquet, Jayne
AU - Barnard, Stephen
AU - Staynova, Albena
AU - Lindholm, Carita
AU - Monteiro Gil, Octávia
AU - Martins, Vanda
AU - Rößler, Ute
AU - Vral, Anne
AU - Vandevoorde, Charlot
AU - Wojewódzka, Maria
AU - Rothkamm, Kai
PY - 2017/1
Y1 - 2017/1
N2 - PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise.MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with (60)Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples.RESULTS: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty.CONCLUSIONS: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
AB - PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise.MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with (60)Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples.RESULTS: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty.CONCLUSIONS: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
U2 - 10.1080/09553002.2016.1207822
DO - 10.1080/09553002.2016.1207822
M3 - SCORING: Journal article
C2 - 27686523
VL - 93
SP - 58
EP - 64
JO - INT J RADIAT BIOL
JF - INT J RADIAT BIOL
SN - 0955-3002
IS - 1
ER -
