Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?

H Thierens; A Vral; C Vandevoorde; V Vandersickel; V de Gelder; H Romm; U Oestreicher; K Rothkamm; S Barnard; E Ainsbury; S Sommer; C Beinke; A Wojcik

doi:10.1093/rpd/ncu130

Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?

Standard

Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? / Thierens, H; Vral, A; Vandevoorde, C; Vandersickel, V; de Gelder, V; Romm, H; Oestreicher, U; Rothkamm, K; Barnard, S; Ainsbury, E; Sommer, S; Beinke, C; Wojcik, A.

In: RADIAT PROT DOSIM, Vol. 159, No. 1-4, 06.2014, p. 87-94.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Thierens, H, Vral, A, Vandevoorde, C, Vandersickel, V, de Gelder, V, Romm, H, Oestreicher, U, Rothkamm, K, Barnard, S, Ainsbury, E, Sommer, S, Beinke, C & Wojcik, A 2014, 'Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?', RADIAT PROT DOSIM, vol. 159, no. 1-4, pp. 87-94. https://doi.org/10.1093/rpd/ncu130

APA

Thierens, H., Vral, A., Vandevoorde, C., Vandersickel, V., de Gelder, V., Romm, H., Oestreicher, U., Rothkamm, K., Barnard, S., Ainsbury, E., Sommer, S., Beinke, C., & Wojcik, A. (2014). Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? RADIAT PROT DOSIM, 159(1-4), 87-94. https://doi.org/10.1093/rpd/ncu130

Vancouver

Thierens H, Vral A, Vandevoorde C, Vandersickel V, de Gelder V, Romm H et al. Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? RADIAT PROT DOSIM. 2014 Jun;159(1-4):87-94. https://doi.org/10.1093/rpd/ncu130

Bibtex

@article{3024f98d72cf4400b51a2f3bdf69a357,

title = "Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?",

abstract = "Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution σ(2)/µ obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay. ",

keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Automation, Computer Simulation, Disaster Planning/organization & administration, Dose-Response Relationship, Radiation, Female, Gamma Rays, Humans, Lymphocytes/radiation effects, Male, Micronucleus Tests/methods, Middle Aged, Phantoms, Imaging, Radiation Injuries/blood, Radiation Monitoring/methods, Triage/methods, Young Adult",

author = "H Thierens and A Vral and C Vandevoorde and V Vandersickel and {de Gelder}, V and H Romm and U Oestreicher and K Rothkamm and S Barnard and E Ainsbury and S Sommer and C Beinke and A Wojcik",

year = "2014",

month = jun,

doi = "10.1093/rpd/ncu130",

language = "English",

volume = "159",

pages = "87--94",

journal = "RADIAT PROT DOSIM",

issn = "0144-8420",

publisher = "Oxford University Press",

number = "1-4",