Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?
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Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? / Thierens, H; Vral, A; Vandevoorde, C; Vandersickel, V; de Gelder, V; Romm, H; Oestreicher, U; Rothkamm, K; Barnard, S; Ainsbury, E; Sommer, S; Beinke, C; Wojcik, A.
in: RADIAT PROT DOSIM, Jahrgang 159, Nr. 1-4, 06.2014, S. 87-94.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?
AU - Thierens, H
AU - Vral, A
AU - Vandevoorde, C
AU - Vandersickel, V
AU - de Gelder, V
AU - Romm, H
AU - Oestreicher, U
AU - Rothkamm, K
AU - Barnard, S
AU - Ainsbury, E
AU - Sommer, S
AU - Beinke, C
AU - Wojcik, A
N1 - © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
PY - 2014/6
Y1 - 2014/6
N2 - Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution σ(2)/µ obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.
AB - Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution σ(2)/µ obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Automation
KW - Computer Simulation
KW - Disaster Planning/organization & administration
KW - Dose-Response Relationship, Radiation
KW - Female
KW - Gamma Rays
KW - Humans
KW - Lymphocytes/radiation effects
KW - Male
KW - Micronucleus Tests/methods
KW - Middle Aged
KW - Phantoms, Imaging
KW - Radiation Injuries/blood
KW - Radiation Monitoring/methods
KW - Triage/methods
KW - Young Adult
U2 - 10.1093/rpd/ncu130
DO - 10.1093/rpd/ncu130
M3 - SCORING: Journal article
C2 - 24743767
VL - 159
SP - 87
EP - 94
JO - RADIAT PROT DOSIM
JF - RADIAT PROT DOSIM
SN - 0144-8420
IS - 1-4
ER -