In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells
Abstract
Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).
Bibliographical data
Original language | English |
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Article number | 100519 |
ISSN | 2666-1667 |
DOIs | |
Publication status | Published - 18.06.2021 |
Externally published | Yes |
Comment Deanary
© 2021 The Author(s).
PubMed | 34036282 |
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