In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

Renée R C E Schreurs; Martin E Baumdick; Agata Drewniak; Madeleine J Bunders

doi:10.1016/j.xpro.2021.100519

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

Standard

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells. / Schreurs, Renée R C E; Baumdick, Martin E; Drewniak, Agata; Bunders, Madeleine J.

In: STAR PROTOC, Vol. 2, No. 2, 100519, 18.06.2021.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schreurs, RRCE, Baumdick, ME, Drewniak, A & Bunders, MJ 2021, 'In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells', STAR PROTOC, vol. 2, no. 2, 100519. https://doi.org/10.1016/j.xpro.2021.100519

APA

Schreurs, R. R. C. E., Baumdick, M. E., Drewniak, A., & Bunders, M. J. (2021). In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells. STAR PROTOC, 2(2), [100519]. https://doi.org/10.1016/j.xpro.2021.100519

Vancouver

Schreurs RRCE, Baumdick ME, Drewniak A, Bunders MJ. In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells. STAR PROTOC. 2021 Jun 18;2(2). 100519. https://doi.org/10.1016/j.xpro.2021.100519

Bibtex

@article{1d38d60bb05846c085eb242aa2a2c76e,

title = "In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells",

abstract = "Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).",

keywords = "CD4-Positive T-Lymphocytes/cytology, Coculture Techniques, Humans, Intestinal Mucosa/cytology, Organoids/cytology",

author = "Schreurs, {Ren{\'e}e R C E} and Baumdick, {Martin E} and Agata Drewniak and Bunders, {Madeleine J}",

note = "{\textcopyright} 2021 The Author(s).",

year = "2021",

month = jun,

day = "18",

doi = "10.1016/j.xpro.2021.100519",

language = "English",

volume = "2",

journal = "STAR PROTOC",

issn = "2666-1667",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

AU - Schreurs, Renée R C E

AU - Baumdick, Martin E

AU - Drewniak, Agata

AU - Bunders, Madeleine J

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).

AB - Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).

KW - CD4-Positive T-Lymphocytes/cytology

KW - Coculture Techniques

KW - Humans

KW - Intestinal Mucosa/cytology

KW - Organoids/cytology

U2 - 10.1016/j.xpro.2021.100519

DO - 10.1016/j.xpro.2021.100519

M3 - SCORING: Journal article

C2 - 34036282

VL - 2

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 2

M1 - 100519

ER -