In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells
Abstract
Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).
Bibliografische Daten
Originalsprache | Englisch |
---|---|
Aufsatznummer | 100519 |
ISSN | 2666-1667 |
DOIs | |
Status | Veröffentlicht - 18.06.2021 |
Extern publiziert | Ja |
Anmerkungen des Dekanats
© 2021 The Author(s).
PubMed | 34036282 |
---|