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Human-Engineered Atrial Tissue for Studying Atrial Fibrillation

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Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. / Krause, Julia; Lemme, Marta; Mannhardt, Ingra; Eder, Alexandra; Ulmer, Bärbel; Eschenhagen, Thomas; Stenzig, Justus.

Cardiac Tissue Engineering: Methods and Protocols. ed. / Kareen L. K. Coulombe; Lauren D. Black III. 1. ed. New York, NY : Humana Press, 2022. p. 159-173 (Methods in Molecular Biology; Vol. 2485).

Research output: SCORING: Contribution to book/anthology › SCORING: Contribution to collected editions/anthologies › Research

Harvard

Krause, J, Lemme, M, Mannhardt, I, Eder, A, Ulmer, B, Eschenhagen, T & Stenzig, J 2022, Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. in KLK Coulombe & LD Black III (eds), Cardiac Tissue Engineering: Methods and Protocols. 1 edn, Methods in Molecular Biology, vol. 2485, Humana Press, New York, NY, pp. 159-173. https://doi.org/10.1007/978-1-0716-2261-2_11

APA

Krause, J., Lemme, M., Mannhardt, I., Eder, A., Ulmer, B., Eschenhagen, T., & Stenzig, J. (2022). Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. In K. L. K. Coulombe, & L. D. Black III (Eds.), Cardiac Tissue Engineering: Methods and Protocols (1 ed., pp. 159-173). (Methods in Molecular Biology; Vol. 2485). Humana Press. https://doi.org/10.1007/978-1-0716-2261-2_11

Vancouver

Krause J, Lemme M, Mannhardt I, Eder A, Ulmer B, Eschenhagen T et al. Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. In Coulombe KLK, Black III LD, editors, Cardiac Tissue Engineering: Methods and Protocols. 1 ed. New York, NY: Humana Press. 2022. p. 159-173. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-2261-2_11

Bibtex

@inbook{0c042d764ec04867b318d9ca2734fb62,
title = "Human-Engineered Atrial Tissue for Studying Atrial Fibrillation",
abstract = "This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.",
author = "Julia Krause and Marta Lemme and Ingra Mannhardt and Alexandra Eder and B{\"a}rbel Ulmer and Thomas Eschenhagen and Justus Stenzig",
note = "{\textcopyright} 2022. Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = may,
day = "27",
doi = "10.1007/978-1-0716-2261-2_11",
language = "English",
isbn = "978-1-0716-2260-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "159--173",
editor = "Coulombe, {Kareen L. K.} and {Black III}, {Lauren D.}",
booktitle = "Cardiac Tissue Engineering",
address = "United States",
edition = "1",

}

RIS

TY - CHAP

T1 - Human-Engineered Atrial Tissue for Studying Atrial Fibrillation

AU - Krause, Julia

AU - Lemme, Marta

AU - Mannhardt, Ingra

AU - Eder, Alexandra

AU - Ulmer, Bärbel

AU - Eschenhagen, Thomas

AU - Stenzig, Justus

N1 - © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022/5/27

Y1 - 2022/5/27

N2 - This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.

AB - This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.

U2 - 10.1007/978-1-0716-2261-2_11

DO - 10.1007/978-1-0716-2261-2_11

M3 - SCORING: Contribution to collected editions/anthologies

C2 - 35618905

SN - 978-1-0716-2260-5

T3 - Methods in Molecular Biology

SP - 159

EP - 173

BT - Cardiac Tissue Engineering

A2 - Coulombe, Kareen L. K.

A2 - Black III, Lauren D.

PB - Humana Press

CY - New York, NY

ER -