Human-Engineered Atrial Tissue for Studying Atrial Fibrillation
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Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. / Krause, Julia; Lemme, Marta; Mannhardt, Ingra; Eder, Alexandra; Ulmer, Bärbel; Eschenhagen, Thomas; Stenzig, Justus.
Cardiac Tissue Engineering: Methods and Protocols. Hrsg. / Kareen L. K. Coulombe; Lauren D. Black III. 1. Aufl. New York, NY : Humana Press, 2022. S. 159-173 (Methods in Molecular Biology; Band 2485).Publikationen: SCORING: Beitrag in Buch/Sammelwerk › SCORING: Beitrag in Sammelwerk › Forschung
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TY - CHAP
T1 - Human-Engineered Atrial Tissue for Studying Atrial Fibrillation
AU - Krause, Julia
AU - Lemme, Marta
AU - Mannhardt, Ingra
AU - Eder, Alexandra
AU - Ulmer, Bärbel
AU - Eschenhagen, Thomas
AU - Stenzig, Justus
N1 - © 2022. Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/5/27
Y1 - 2022/5/27
N2 - This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.
AB - This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.
U2 - 10.1007/978-1-0716-2261-2_11
DO - 10.1007/978-1-0716-2261-2_11
M3 - SCORING: Contribution to collected editions/anthologies
C2 - 35618905
SN - 978-1-0716-2260-5
T3 - Methods in Molecular Biology
SP - 159
EP - 173
BT - Cardiac Tissue Engineering
A2 - Coulombe, Kareen L. K.
A2 - Black III, Lauren D.
PB - Humana Press
CY - New York, NY
ER -