Human-Engineered Atrial Tissue for Studying Atrial Fibrillation

  • Julia Krause
  • Marta Lemme
  • Ingra Mannhardt
  • Alexandra Eder
  • Bärbel Ulmer
  • Thomas Eschenhagen
  • Justus Stenzig

Abstract

This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.

Bibliographical data

Original languageEnglish
Title of host publicationCardiac Tissue Engineering : Methods and Protocols
EditorsKareen L. K. Coulombe, Lauren D. Black III
REQUIRED books only: Number of pages15
Place of PublicationNew York, NY
PublisherHumana Press
Publication date27.05.2022
Edition1
Pages159-173
ISBN (Print)978-1-0716-2260-5
ISBN (Electronic)978-1-0716-2261-2
DOIs
Publication statusPublished - 27.05.2022

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PubMed 35618905