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gamma-H2AX as protein biomarker for radiation exposure

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gamma-H2AX as protein biomarker for radiation exposure. / Rothkamm, Kai; Horn, Simon.

In: ANN I SUPER SANITA, Vol. 45, No. 3, 2009, p. 265-71.

Research output: SCORING: Contribution to journal › SCORING: Review article › Research

Harvard

Rothkamm, K & Horn, S 2009, 'gamma-H2AX as protein biomarker for radiation exposure', ANN I SUPER SANITA, vol. 45, no. 3, pp. 265-71.

APA

Rothkamm, K., & Horn, S. (2009). gamma-H2AX as protein biomarker for radiation exposure. ANN I SUPER SANITA, 45(3), 265-71.

Vancouver

Rothkamm K, Horn S. gamma-H2AX as protein biomarker for radiation exposure. ANN I SUPER SANITA. 2009;45(3):265-71.

Bibtex

@article{607f38b416ba48aa83014eb1f028cabf,
title = "gamma-H2AX as protein biomarker for radiation exposure",
abstract = "For large scale exposures of the human population to ionising radiation, there is a need for cost-effective high throughput assessment of radiation exposure levels from biological samples to allow triage decisions to be made. Here we discuss the usefulness of the DNA damage marker gamma-H2AX for this purpose. Foci of gamma-H2AX form in response to radiation-induced DNA doublestrand breaks and can be quantified by immunofluorescence microscopy or flow cytometry. Several studies have analysed this marker in patients' blood samples to determine radiation exposures during various diagnostic or therapeutic radiation treatments. Such planned exposures involve only a moderate number of samples which can be obtained at a prearranged time following exposure. In contrast, application of this method as a triage tool in large scale radiological emergencies demands high throughput sample processing and analysis. The rapid kinetics of gamma-H2AX induction and loss presents a major challenge to its successful application as a triage tool. These and other as yet unresolved questions are discussed.",
keywords = "Biomarkers, DNA Breaks, Double-Stranded/radiation effects, Flow Cytometry, Histones/blood, Humans, Microscopy, Fluorescence, Phosphorylation, Radiation Injuries/blood",
author = "Kai Rothkamm and Simon Horn",
year = "2009",
language = "English",
volume = "45",
pages = "265--71",
journal = "ANN I SUPER SANITA",
issn = "0021-2571",
publisher = "Istituto Superiore di Sanita",
number = "3",

}

RIS

TY - JOUR

T1 - gamma-H2AX as protein biomarker for radiation exposure

AU - Rothkamm, Kai

AU - Horn, Simon

PY - 2009

Y1 - 2009

N2 - For large scale exposures of the human population to ionising radiation, there is a need for cost-effective high throughput assessment of radiation exposure levels from biological samples to allow triage decisions to be made. Here we discuss the usefulness of the DNA damage marker gamma-H2AX for this purpose. Foci of gamma-H2AX form in response to radiation-induced DNA doublestrand breaks and can be quantified by immunofluorescence microscopy or flow cytometry. Several studies have analysed this marker in patients' blood samples to determine radiation exposures during various diagnostic or therapeutic radiation treatments. Such planned exposures involve only a moderate number of samples which can be obtained at a prearranged time following exposure. In contrast, application of this method as a triage tool in large scale radiological emergencies demands high throughput sample processing and analysis. The rapid kinetics of gamma-H2AX induction and loss presents a major challenge to its successful application as a triage tool. These and other as yet unresolved questions are discussed.

AB - For large scale exposures of the human population to ionising radiation, there is a need for cost-effective high throughput assessment of radiation exposure levels from biological samples to allow triage decisions to be made. Here we discuss the usefulness of the DNA damage marker gamma-H2AX for this purpose. Foci of gamma-H2AX form in response to radiation-induced DNA doublestrand breaks and can be quantified by immunofluorescence microscopy or flow cytometry. Several studies have analysed this marker in patients' blood samples to determine radiation exposures during various diagnostic or therapeutic radiation treatments. Such planned exposures involve only a moderate number of samples which can be obtained at a prearranged time following exposure. In contrast, application of this method as a triage tool in large scale radiological emergencies demands high throughput sample processing and analysis. The rapid kinetics of gamma-H2AX induction and loss presents a major challenge to its successful application as a triage tool. These and other as yet unresolved questions are discussed.

KW - Biomarkers

KW - DNA Breaks, Double-Stranded/radiation effects

KW - Flow Cytometry

KW - Histones/blood

KW - Humans

KW - Microscopy, Fluorescence

KW - Phosphorylation

KW - Radiation Injuries/blood

M3 - SCORING: Review article

C2 - 19861731

VL - 45

SP - 265

EP - 271

JO - ANN I SUPER SANITA

JF - ANN I SUPER SANITA

SN - 0021-2571

IS - 3

ER -