Capture of viable circulating tumor cells in the liver of colorectal cancer patients
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Capture of viable circulating tumor cells in the liver of colorectal cancer patients. / Denève, Eric; Riethdorf, Sabine; Ramos, Jeanne; Nocca, David; Coffy, Amandine; Daurès, Jean-Pierre; Maudelonde, Thierry; Fabre, Jean-Michel; Pantel, Klaus; Alix-Panabières, Catherine.
In: CLIN CHEM, Vol. 59, No. 9, 01.09.2013, p. 1384-92.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Capture of viable circulating tumor cells in the liver of colorectal cancer patients
AU - Denève, Eric
AU - Riethdorf, Sabine
AU - Ramos, Jeanne
AU - Nocca, David
AU - Coffy, Amandine
AU - Daurès, Jean-Pierre
AU - Maudelonde, Thierry
AU - Fabre, Jean-Michel
AU - Pantel, Klaus
AU - Alix-Panabières, Catherine
PY - 2013/9/1
Y1 - 2013/9/1
N2 - BACKGROUND: The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.METHODS: We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.RESULTS: The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).CONCLUSIONS: A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.
AB - BACKGROUND: The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.METHODS: We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.RESULTS: The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).CONCLUSIONS: A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Colon
KW - Colorectal Neoplasms
KW - Female
KW - Humans
KW - Liver
KW - Male
KW - Middle Aged
KW - Neoplastic Cells, Circulating
KW - Prognosis
KW - Rectum
U2 - 10.1373/clinchem.2013.202846
DO - 10.1373/clinchem.2013.202846
M3 - SCORING: Journal article
C2 - 23695297
VL - 59
SP - 1384
EP - 1392
JO - CLIN CHEM
JF - CLIN CHEM
SN - 0009-9147
IS - 9
ER -