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Capture of viable circulating tumor cells in the liver of colorectal cancer patients

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Capture of viable circulating tumor cells in the liver of colorectal cancer patients. / Denève, Eric; Riethdorf, Sabine; Ramos, Jeanne; Nocca, David; Coffy, Amandine; Daurès, Jean-Pierre; Maudelonde, Thierry; Fabre, Jean-Michel; Pantel, Klaus; Alix-Panabières, Catherine.

in: CLIN CHEM, Jahrgang 59, Nr. 9, 01.09.2013, S. 1384-92.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Denève, E, Riethdorf, S, Ramos, J, Nocca, D, Coffy, A, Daurès, J-P, Maudelonde, T, Fabre, J-M, Pantel, K & Alix-Panabières, C 2013, 'Capture of viable circulating tumor cells in the liver of colorectal cancer patients', CLIN CHEM, Jg. 59, Nr. 9, S. 1384-92. https://doi.org/10.1373/clinchem.2013.202846

APA

Denève, E., Riethdorf, S., Ramos, J., Nocca, D., Coffy, A., Daurès, J-P., Maudelonde, T., Fabre, J-M., Pantel, K., & Alix-Panabières, C. (2013). Capture of viable circulating tumor cells in the liver of colorectal cancer patients. CLIN CHEM, 59(9), 1384-92. https://doi.org/10.1373/clinchem.2013.202846

Vancouver

Denève E, Riethdorf S, Ramos J, Nocca D, Coffy A, Daurès J-P et al. Capture of viable circulating tumor cells in the liver of colorectal cancer patients. CLIN CHEM. 2013 Sep 1;59(9):1384-92. https://doi.org/10.1373/clinchem.2013.202846

Bibtex

@article{0dc25724c6e54a3d98c5bd2457052bab,
title = "Capture of viable circulating tumor cells in the liver of colorectal cancer patients",
abstract = "BACKGROUND: The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.METHODS: We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch{\textregistered} as the reference method.RESULTS: The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).CONCLUSIONS: A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.",
keywords = "Adult, Aged, Aged, 80 and over, Colon, Colorectal Neoplasms, Female, Humans, Liver, Male, Middle Aged, Neoplastic Cells, Circulating, Prognosis, Rectum",
author = "Eric Den{\`e}ve and Sabine Riethdorf and Jeanne Ramos and David Nocca and Amandine Coffy and Jean-Pierre Daur{\`e}s and Thierry Maudelonde and Jean-Michel Fabre and Klaus Pantel and Catherine Alix-Panabi{\`e}res",
year = "2013",
month = sep,
day = "1",
doi = "10.1373/clinchem.2013.202846",
language = "English",
volume = "59",
pages = "1384--92",
journal = "CLIN CHEM",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "9",

}

RIS

TY - JOUR

T1 - Capture of viable circulating tumor cells in the liver of colorectal cancer patients

AU - Denève, Eric

AU - Riethdorf, Sabine

AU - Ramos, Jeanne

AU - Nocca, David

AU - Coffy, Amandine

AU - Daurès, Jean-Pierre

AU - Maudelonde, Thierry

AU - Fabre, Jean-Michel

AU - Pantel, Klaus

AU - Alix-Panabières, Catherine

PY - 2013/9/1

Y1 - 2013/9/1

N2 - BACKGROUND: The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.METHODS: We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.RESULTS: The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).CONCLUSIONS: A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.

AB - BACKGROUND: The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.METHODS: We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.RESULTS: The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).CONCLUSIONS: A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Colon

KW - Colorectal Neoplasms

KW - Female

KW - Humans

KW - Liver

KW - Male

KW - Middle Aged

KW - Neoplastic Cells, Circulating

KW - Prognosis

KW - Rectum

U2 - 10.1373/clinchem.2013.202846

DO - 10.1373/clinchem.2013.202846

M3 - SCORING: Journal article

C2 - 23695297

VL - 59

SP - 1384

EP - 1392

JO - CLIN CHEM

JF - CLIN CHEM

SN - 0009-9147

IS - 9

ER -