A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.

Sujoy Bera; raghuram vijeta; Marina Mikhaylova; Michael R  Kreutz

doi:10.1016

A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.

Standard

A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo. / Bera, Sujoy; vijeta, raghuram; Mikhaylova, Marina; Kreutz, Michael R .

In: ANAL BIOCHEM, Vol. 502, 01.06.2016, p. 50-52.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bera, S, vijeta, R, Mikhaylova, M & Kreutz, MR 2016, 'A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.', ANAL BIOCHEM, vol. 502, pp. 50-52. https://doi.org/10.1016

APA

Bera, S., vijeta, R., Mikhaylova, M., & Kreutz, M. R. (2016). A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo. ANAL BIOCHEM, 502, 50-52. https://doi.org/10.1016

Vancouver

Bera S, vijeta R, Mikhaylova M, Kreutz MR. A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo. ANAL BIOCHEM. 2016 Jun 1;502:50-52. https://doi.org/10.1016

Bibtex

@article{c08577288661472284319328a678cd0e,

title = "A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.",

abstract = "There is still an unmet need for simple methods to verify, visualize, and confirm protein–protein interactions in vivo. Here we describe a plasmid-based system to study such interactions. The system is based on the transmembrane domain (TMD) of the EF-hand Ca2+ sensor protein calneuron-2. We show that fusion of 28 amino acids that include the TMD of calneuron-2 to proteins of interest results in prominent localization on the cytoplasmic side of the Golgi. The recruitment of binding partners to the protein of interest fused to this sequence can then be easily visualized by fluorescent tags.",

author = "Sujoy Bera and raghuram vijeta and Marina Mikhaylova and Kreutz, {Michael R}",

year = "2016",

month = jun,

day = "1",

doi = "10.1016",

language = "English",

volume = "502",

pages = "50--52",

journal = "ANAL BIOCHEM",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.

AU - Bera, Sujoy

AU - vijeta, raghuram

AU - Mikhaylova, Marina

AU - Kreutz, Michael R

PY - 2016/6/1

Y1 - 2016/6/1

N2 - There is still an unmet need for simple methods to verify, visualize, and confirm protein–protein interactions in vivo. Here we describe a plasmid-based system to study such interactions. The system is based on the transmembrane domain (TMD) of the EF-hand Ca2+ sensor protein calneuron-2. We show that fusion of 28 amino acids that include the TMD of calneuron-2 to proteins of interest results in prominent localization on the cytoplasmic side of the Golgi. The recruitment of binding partners to the protein of interest fused to this sequence can then be easily visualized by fluorescent tags.

AB - There is still an unmet need for simple methods to verify, visualize, and confirm protein–protein interactions in vivo. Here we describe a plasmid-based system to study such interactions. The system is based on the transmembrane domain (TMD) of the EF-hand Ca2+ sensor protein calneuron-2. We show that fusion of 28 amino acids that include the TMD of calneuron-2 to proteins of interest results in prominent localization on the cytoplasmic side of the Golgi. The recruitment of binding partners to the protein of interest fused to this sequence can then be easily visualized by fluorescent tags.

U2 - 10.1016

DO - 10.1016

M3 - SCORING: Journal article

VL - 502

SP - 50

EP - 52

JO - ANAL BIOCHEM

JF - ANAL BIOCHEM

SN - 0003-2697

ER -