A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.
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A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo. / Bera, Sujoy; vijeta, raghuram; Mikhaylova, Marina; Kreutz, Michael R .
in: ANAL BIOCHEM, Jahrgang 502, 01.06.2016, S. 50-52.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A plasmid-based expression system to study protein-protein interactions at the Golgi in vivo.
AU - Bera, Sujoy
AU - vijeta, raghuram
AU - Mikhaylova, Marina
AU - Kreutz, Michael R
PY - 2016/6/1
Y1 - 2016/6/1
N2 - There is still an unmet need for simple methods to verify, visualize, and confirm protein–protein interactions in vivo. Here we describe a plasmid-based system to study such interactions. The system is based on the transmembrane domain (TMD) of the EF-hand Ca2+ sensor protein calneuron-2. We show that fusion of 28 amino acids that include the TMD of calneuron-2 to proteins of interest results in prominent localization on the cytoplasmic side of the Golgi. The recruitment of binding partners to the protein of interest fused to this sequence can then be easily visualized by fluorescent tags.
AB - There is still an unmet need for simple methods to verify, visualize, and confirm protein–protein interactions in vivo. Here we describe a plasmid-based system to study such interactions. The system is based on the transmembrane domain (TMD) of the EF-hand Ca2+ sensor protein calneuron-2. We show that fusion of 28 amino acids that include the TMD of calneuron-2 to proteins of interest results in prominent localization on the cytoplasmic side of the Golgi. The recruitment of binding partners to the protein of interest fused to this sequence can then be easily visualized by fluorescent tags.
U2 - 10.1016
DO - 10.1016
M3 - SCORING: Journal article
VL - 502
SP - 50
EP - 52
JO - ANAL BIOCHEM
JF - ANAL BIOCHEM
SN - 0003-2697
ER -