A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells
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A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells. / Berger, Carolina; Berger, M; Anderson, D; Riddell, S R.
In: J MED PRIMATOL, Vol. 40, No. 2, 04.2011, p. 88-103.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells
AU - Berger, Carolina
AU - Berger, M
AU - Anderson, D
AU - Riddell, S R
N1 - © 2010 John Wiley & Sons A/S.
PY - 2011/4
Y1 - 2011/4
N2 - BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.
AB - BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.
KW - Animals
KW - Antigens, CD19/immunology
KW - Antigens, CD20/immunology
KW - CD8-Positive T-Lymphocytes/immunology
KW - Cell Culture Techniques
KW - Cell Movement
KW - Cell Survival
KW - Cells, Cultured
KW - Cytomegalovirus/immunology
KW - Cytomegalovirus Infections/immunology
KW - Immunologic Memory
KW - Immunotherapy, Adoptive
KW - Macaca nemestrina/immunology
KW - Models, Animal
KW - Neoplasms/immunology
KW - Polymerase Chain Reaction
U2 - 10.1111/j.1600-0684.2010.00451.x
DO - 10.1111/j.1600-0684.2010.00451.x
M3 - SCORING: Journal article
C2 - 21044089
VL - 40
SP - 88
EP - 103
JO - J MED PRIMATOL
JF - J MED PRIMATOL
SN - 0047-2565
IS - 2
ER -