A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells
Abstract
BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.
METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.
RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.
CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.
Bibliographical data
Original language | English |
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ISSN | 0047-2565 |
DOIs | |
Publication status | Published - 04.2011 |
Externally published | Yes |
Comment Deanary
© 2010 John Wiley & Sons A/S.
PubMed | 21044089 |
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