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A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells

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A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells. / Berger, Carolina; Berger, M; Anderson, D; Riddell, S R.

in: J MED PRIMATOL, Jahrgang 40, Nr. 2, 04.2011, S. 88-103.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Berger, C, Berger, M, Anderson, D & Riddell, SR 2011, 'A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells', J MED PRIMATOL, Jg. 40, Nr. 2, S. 88-103. https://doi.org/10.1111/j.1600-0684.2010.00451.x

APA

Berger, C., Berger, M., Anderson, D., & Riddell, S. R. (2011). A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells. J MED PRIMATOL, 40(2), 88-103. https://doi.org/10.1111/j.1600-0684.2010.00451.x

Vancouver

Berger C, Berger M, Anderson D, Riddell SR. A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells. J MED PRIMATOL. 2011 Apr;40(2):88-103. https://doi.org/10.1111/j.1600-0684.2010.00451.x

Bibtex

@article{6a68545bf8a843a8ae7899415c0a1ce8,
title = "A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells",
abstract = "BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.",
keywords = "Animals, Antigens, CD19/immunology, Antigens, CD20/immunology, CD8-Positive T-Lymphocytes/immunology, Cell Culture Techniques, Cell Movement, Cell Survival, Cells, Cultured, Cytomegalovirus/immunology, Cytomegalovirus Infections/immunology, Immunologic Memory, Immunotherapy, Adoptive, Macaca nemestrina/immunology, Models, Animal, Neoplasms/immunology, Polymerase Chain Reaction",
author = "Carolina Berger and M Berger and D Anderson and Riddell, {S R}",
note = "{\textcopyright} 2010 John Wiley & Sons A/S.",
year = "2011",
month = apr,
doi = "10.1111/j.1600-0684.2010.00451.x",
language = "English",
volume = "40",
pages = "88--103",
journal = "J MED PRIMATOL",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "2",

}

RIS

TY - JOUR

T1 - A non-human primate model for analysis of safety, persistence, and function of adoptively transferred T cells

AU - Berger, Carolina

AU - Berger, M

AU - Anderson, D

AU - Riddell, S R

N1 - © 2010 John Wiley & Sons A/S.

PY - 2011/4

Y1 - 2011/4

N2 - BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.

AB - BACKGROUND: Adoptive immunotherapy with antigen-specific effector T-cell (T(E) ) clones is often limited by poor survival of the transferred cells. We describe here a Macaca nemestrina model for studying transfer of T-cell immunity.METHODS: We derived, expanded, and genetically marked CMV-specific CD8(+) T(E) clones with surface markers expressed on B cells. T(E) cells were adoptively transferred, and toxicity, persistence, retention of introduced cell-surface markers, and phenotype of the persisting T cells were evaluated.RESULTS: CD8(+) T(E) clones were efficiently isolated from distinct memory precursors and gene-marking with CD19 or CD20 permitted in vivo tracking by quantitative PCR. CD19 was a more stable surface marker for tracking cells in vivo and was used to re-isolate cells for functional analysis. Clonally derived CD8(+) T(E) cells differentiated in vivo to phenotypically and functionally heterogeneous memory T-cell subsets.CONCLUSIONS: These studies demonstrate the utility of Macaca nemestrina for establishing principles for T-cell therapeutics applicable to humans.

KW - Animals

KW - Antigens, CD19/immunology

KW - Antigens, CD20/immunology

KW - CD8-Positive T-Lymphocytes/immunology

KW - Cell Culture Techniques

KW - Cell Movement

KW - Cell Survival

KW - Cells, Cultured

KW - Cytomegalovirus/immunology

KW - Cytomegalovirus Infections/immunology

KW - Immunologic Memory

KW - Immunotherapy, Adoptive

KW - Macaca nemestrina/immunology

KW - Models, Animal

KW - Neoplasms/immunology

KW - Polymerase Chain Reaction

U2 - 10.1111/j.1600-0684.2010.00451.x

DO - 10.1111/j.1600-0684.2010.00451.x

M3 - SCORING: Journal article

C2 - 21044089

VL - 40

SP - 88

EP - 103

JO - J MED PRIMATOL

JF - J MED PRIMATOL

SN - 0047-2565

IS - 2

ER -