Stefan Linder

Our group is interested in the cytoskeleton of primary human cells, especially of monocytic and endothelial cells. We study the dynamic regulation of the actin and tubulin cytoskeletons in the context of cell adhesion, migration, invasion and phagocytosis. These cellular functions are based on closely regulated and spatially restricted reorganization of the cytoskeleton, and play critical roles during physiological or pathological processes such as diapedesis, wound healing, metastasis, or uptake of pathogens.

A detailed study of the molecular mechanisms underlying cytoskeletal dynamics is, therefore, essential for a better understanding of these processes and for the development of specific tools for their therapeutic modulation. Our projects focus on various model systems, which address different aspects of cell adhesion, migration, invasion or phagocytosis, and are all critically regulated by actin and tubulin turnover.

Macrophage adhesion and migration
Influence of actin-associated proteins in the turnover of podosomal adhesions.

Cytoskeletal cooperation at podosomes
Dynamic regulation of actin-dependent podosomes by microtubules and molecular motor proteins.

Regulation of cell invasion
Intracellular trafficking of matrix metalloproteinases and their influence on extracellular matrix degradation.

Processing of pathogens by immune cells
Uptake and intracellular handling of Borrelia burgdorferi by human macrophages and dendritic cells.

Regulation of endothelial integrity
Role of RhoGTPase effectors and actin-associated proteins in the dynamic formation of cell-cell contacts.