Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo
Standard
Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo. / Demais, Valerie; Pohl, Anne; Wunderlich, Kirsten A.; Pfaller, Anna M.; Kaplan, Lew; Barthélémy, Amelie; Dittrich, Robin; Puig, Berta; Giebel, Bernd; Hauck, Stefanie M.; Pfrieger, Frank W.; Grosche, Antje.
in: J EXTRACELL VESICLES, Jahrgang 11, Nr. 9, 09.2022, S. e12254.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo
AU - Demais, Valerie
AU - Pohl, Anne
AU - Wunderlich, Kirsten A.
AU - Pfaller, Anna M.
AU - Kaplan, Lew
AU - Barthélémy, Amelie
AU - Dittrich, Robin
AU - Puig, Berta
AU - Giebel, Bernd
AU - Hauck, Stefanie M.
AU - Pfrieger, Frank W.
AU - Grosche, Antje
N1 - © 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
PY - 2022/9
Y1 - 2022/9
N2 - Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Müller cells in adult mice in vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Müller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.
AB - Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Müller cells in adult mice in vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Müller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.
KW - Animals
KW - Ependymoglial Cells/metabolism
KW - Extracellular Vesicles
KW - Mice
KW - Neuroglia/metabolism
KW - Neurons/metabolism
KW - Retina/metabolism
U2 - 10.1002/jev2.12254
DO - 10.1002/jev2.12254
M3 - SCORING: Journal article
C2 - 36043482
VL - 11
SP - e12254
JO - J EXTRACELL VESICLES
JF - J EXTRACELL VESICLES
SN - 2001-3078
IS - 9
ER -