Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo

Valerie Demais; Anne Pohl; Kirsten A. Wunderlich; Anna M. Pfaller; Lew Kaplan; Amelie Barthélémy; Robin Dittrich; Berta Puig; Bernd Giebel; Stefanie M. Hauck; Frank W. Pfrieger; Antje Grosche

doi:10.1002/jev2.12254

Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo

Standard

Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo. / Demais, Valerie; Pohl, Anne; Wunderlich, Kirsten A.; Pfaller, Anna M.; Kaplan, Lew; Barthélémy, Amelie; Dittrich, Robin; Puig, Berta; Giebel, Bernd; Hauck, Stefanie M.; Pfrieger, Frank W.; Grosche, Antje.

In: J EXTRACELL VESICLES, Vol. 11, No. 9, 09.2022, p. e12254.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Demais, V, Pohl, A, Wunderlich, KA, Pfaller, AM, Kaplan, L, Barthélémy, A, Dittrich, R, Puig, B, Giebel, B, Hauck, SM, Pfrieger, FW & Grosche, A 2022, 'Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo', J EXTRACELL VESICLES, vol. 11, no. 9, pp. e12254. https://doi.org/10.1002/jev2.12254

APA

Demais, V., Pohl, A., Wunderlich, K. A., Pfaller, A. M., Kaplan, L., Barthélémy, A., Dittrich, R., Puig, B., Giebel, B., Hauck, S. M., Pfrieger, F. W., & Grosche, A. (2022). Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo. J EXTRACELL VESICLES, 11(9), e12254. https://doi.org/10.1002/jev2.12254

Vancouver

Demais V, Pohl A, Wunderlich KA, Pfaller AM, Kaplan L, Barthélémy A et al. Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo. J EXTRACELL VESICLES. 2022 Sep;11(9):e12254. https://doi.org/10.1002/jev2.12254

Bibtex

@article{c0b089e41a494e7c81688c8866b2d260,

title = "Release of VAMP5-positive extracellular vesicles by retinal M{\"u}ller glia in vivo",

abstract = "Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by M{\"u}ller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal M{\"u}ller cells in adult mice in vivo. We identify VAMP5 as a M{\"u}ller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified M{\"u}ller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.",

keywords = "Animals, Ependymoglial Cells/metabolism, Extracellular Vesicles, Mice, Neuroglia/metabolism, Neurons/metabolism, Retina/metabolism",

author = "Valerie Demais and Anne Pohl and Wunderlich, {Kirsten A.} and Pfaller, {Anna M.} and Lew Kaplan and Amelie Barth{\'e}l{\'e}my and Robin Dittrich and Berta Puig and Bernd Giebel and Hauck, {Stefanie M.} and Pfrieger, {Frank W.} and Antje Grosche",

note = "{\textcopyright} 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.",

year = "2022",

month = sep,

doi = "10.1002/jev2.12254",

language = "English",

volume = "11",

pages = "e12254",

journal = "J EXTRACELL VESICLES",

issn = "2001-3078",

publisher = "Taylor and Francis Ltd.",

number = "9",

}

RIS

TY - JOUR

T1 - Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo

AU - Demais, Valerie

AU - Pohl, Anne

AU - Wunderlich, Kirsten A.

AU - Pfaller, Anna M.

AU - Kaplan, Lew

AU - Barthélémy, Amelie

AU - Dittrich, Robin

AU - Puig, Berta

AU - Giebel, Bernd

AU - Hauck, Stefanie M.

AU - Pfrieger, Frank W.

AU - Grosche, Antje

PY - 2022/9

Y1 - 2022/9

N2 - Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Müller cells in adult mice in vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Müller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.

AB - Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Müller cells in adult mice in vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Müller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.

KW - Animals

KW - Ependymoglial Cells/metabolism

KW - Extracellular Vesicles

KW - Mice

KW - Neuroglia/metabolism

KW - Neurons/metabolism

KW - Retina/metabolism

U2 - 10.1002/jev2.12254

DO - 10.1002/jev2.12254

M3 - SCORING: Journal article

C2 - 36043482

VL - 11

SP - e12254

JO - J EXTRACELL VESICLES

JF - J EXTRACELL VESICLES

SN - 2001-3078

IS - 9

ER -