Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

TY - JOUR

T1 - Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

AU - Welsh, Joshua A

AU - Goberdhan, Deborah C I

AU - O'Driscoll, Lorraine

AU - Buzas, Edit I

AU - Blenkiron, Cherie

AU - Bussolati, Benedetta

AU - Cai, Houjian

AU - Di Vizio, Dolores

AU - Driedonks, Tom A P

AU - Erdbrügger, Uta

AU - Falcon-Perez, Juan M

AU - Fu, Qing-Ling

AU - Hill, Andrew F

AU - Lenassi, Metka

AU - Lim, Sai Kiang

AU - Mahoney, Mỹ G

AU - Mohanty, Sujata

AU - Möller, Andreas

AU - Nieuwland, Rienk

AU - Ochiya, Takahiro

AU - Sahoo, Susmita

AU - Torrecilhas, Ana C

AU - Zheng, Lei

AU - Zijlstra, Andries

AU - Abuelreich, Sarah

AU - Bagabas, Reem

AU - Bergese, Paolo

AU - Bridges, Esther M

AU - Brucale, Marco

AU - Burger, Dylan

AU - Carney, Randy P

AU - Cocucci, Emanuele

AU - Crescitelli, Rossella

AU - Hanser, Edveena

AU - Harris, Adrian L

AU - Haughey, Norman J

AU - Hendrix, An

AU - Ivanov, Alexander R

AU - Jovanovic-Talisman, Tijana

AU - Kruh-Garcia, Nicole A

AU - Ku'ulei-Lyn Faustino, Vroniqa

AU - Kyburz, Diego

AU - Lässer, Cecilia

AU - Lennon, Kathleen M

AU - Lötvall, Jan

AU - Maddox, Adam L

AU - Martens-Uzunova, Elena S

AU - Mizenko, Rachel R

AU - Newman, Lauren A

AU - Ridolfi, Andrea

AU - Rohde, Eva

AU - Rojalin, Tatu

AU - Rowland, Andrew

AU - Saftics, Andras

AU - Sandau, Ursula S

AU - Saugstad, Julie A

AU - Shekari, Faezeh

AU - Swift, Simon

AU - Ter-Ovanesyan, Dmitry

AU - Tosar, Juan P

AU - Useckaite, Zivile

AU - Valle, Francesco

AU - Varga, Zoltan

AU - van der Pol, Edwin

AU - van Herwijnen, Martijn J C

AU - Wauben, Marca H M

AU - Wehman, Ann M

AU - Williams, Sarah

AU - Zendrini, Andrea

AU - Zimmerman, Alan J

AU - Théry, Clotilde

AU - Witwer, Kenneth W

AU - MISEV Consortium

AU - Braun, Fabian

AU - Brenna, Santra

AU - Krasemann, Susanne

AU - Matamoros Angles, Andreu

AU - Ricklefs, Franz Lennard

AU - Salviano da Silva, Amanda

AU - Saul, Meike J.

N1 - © 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.

PY - 2024/2

Y1 - 2024/2

N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

KW - Extracellular Vesicles/metabolism

KW - Exosomes/metabolism

KW - Biological Transport

KW - Biomarkers/metabolism

KW - Phenotype

U2 - 10.1002/jev2.12404

DO - 10.1002/jev2.12404

M3 - Guideline, recommendation, statement

C2 - 38326288

VL - 13

SP - e12404

JO - J EXTRACELL VESICLES

JF - J EXTRACELL VESICLES

SN - 2001-3078

IS - 2

ER -