Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. / Welsh, Joshua A; Goberdhan, Deborah C I; O'Driscoll, Lorraine; Buzas, Edit I; Blenkiron, Cherie; Bussolati, Benedetta; Cai, Houjian; Di Vizio, Dolores; Driedonks, Tom A P; Erdbrügger, Uta; Falcon-Perez, Juan M; Fu, Qing-Ling; Hill, Andrew F; Lenassi, Metka; Lim, Sai Kiang; Mahoney, Mỹ G; Mohanty, Sujata; Möller, Andreas; Nieuwland, Rienk; Ochiya, Takahiro; Sahoo, Susmita; Torrecilhas, Ana C; Zheng, Lei; Zijlstra, Andries; Abuelreich, Sarah; Bagabas, Reem; Bergese, Paolo; Bridges, Esther M; Brucale, Marco; Burger, Dylan; Carney, Randy P; Cocucci, Emanuele; Crescitelli, Rossella; Hanser, Edveena; Harris, Adrian L; Haughey, Norman J; Hendrix, An; Ivanov, Alexander R; Jovanovic-Talisman, Tijana; Kruh-Garcia, Nicole A; Ku'ulei-Lyn Faustino, Vroniqa; Kyburz, Diego; Lässer, Cecilia; Lennon, Kathleen M; Lötvall, Jan; Maddox, Adam L; Martens-Uzunova, Elena S; Mizenko, Rachel R; Newman, Lauren A; Ridolfi, Andrea; Rohde, Eva; Rojalin, Tatu; Rowland, Andrew; Saftics, Andras; Sandau, Ursula S; Saugstad, Julie A; Shekari, Faezeh; Swift, Simon; Ter-Ovanesyan, Dmitry; Tosar, Juan P; Useckaite, Zivile; Valle, Francesco; Varga, Zoltan; van der Pol, Edwin; van Herwijnen, Martijn J C; Wauben, Marca H M; Wehman, Ann M; Williams, Sarah; Zendrini, Andrea; Zimmerman, Alan J; Théry, Clotilde; Witwer, Kenneth W; MISEV Consortium.
In: J EXTRACELL VESICLES, Vol. 13, No. 2, 02.2024, p. e12404.Research output: SCORING: Contribution to journal › Guideline, recommendation, statement › Research › peer-review
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T1 - Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
AU - Welsh, Joshua A
AU - Goberdhan, Deborah C I
AU - O'Driscoll, Lorraine
AU - Buzas, Edit I
AU - Blenkiron, Cherie
AU - Bussolati, Benedetta
AU - Cai, Houjian
AU - Di Vizio, Dolores
AU - Driedonks, Tom A P
AU - Erdbrügger, Uta
AU - Falcon-Perez, Juan M
AU - Fu, Qing-Ling
AU - Hill, Andrew F
AU - Lenassi, Metka
AU - Lim, Sai Kiang
AU - Mahoney, Mỹ G
AU - Mohanty, Sujata
AU - Möller, Andreas
AU - Nieuwland, Rienk
AU - Ochiya, Takahiro
AU - Sahoo, Susmita
AU - Torrecilhas, Ana C
AU - Zheng, Lei
AU - Zijlstra, Andries
AU - Abuelreich, Sarah
AU - Bagabas, Reem
AU - Bergese, Paolo
AU - Bridges, Esther M
AU - Brucale, Marco
AU - Burger, Dylan
AU - Carney, Randy P
AU - Cocucci, Emanuele
AU - Crescitelli, Rossella
AU - Hanser, Edveena
AU - Harris, Adrian L
AU - Haughey, Norman J
AU - Hendrix, An
AU - Ivanov, Alexander R
AU - Jovanovic-Talisman, Tijana
AU - Kruh-Garcia, Nicole A
AU - Ku'ulei-Lyn Faustino, Vroniqa
AU - Kyburz, Diego
AU - Lässer, Cecilia
AU - Lennon, Kathleen M
AU - Lötvall, Jan
AU - Maddox, Adam L
AU - Martens-Uzunova, Elena S
AU - Mizenko, Rachel R
AU - Newman, Lauren A
AU - Ridolfi, Andrea
AU - Rohde, Eva
AU - Rojalin, Tatu
AU - Rowland, Andrew
AU - Saftics, Andras
AU - Sandau, Ursula S
AU - Saugstad, Julie A
AU - Shekari, Faezeh
AU - Swift, Simon
AU - Ter-Ovanesyan, Dmitry
AU - Tosar, Juan P
AU - Useckaite, Zivile
AU - Valle, Francesco
AU - Varga, Zoltan
AU - van der Pol, Edwin
AU - van Herwijnen, Martijn J C
AU - Wauben, Marca H M
AU - Wehman, Ann M
AU - Williams, Sarah
AU - Zendrini, Andrea
AU - Zimmerman, Alan J
AU - Théry, Clotilde
AU - Witwer, Kenneth W
AU - MISEV Consortium
AU - Braun, Fabian
AU - Brenna, Santra
AU - Krasemann, Susanne
AU - Matamoros Angles, Andreu
AU - Ricklefs, Franz Lennard
AU - Salviano da Silva, Amanda
AU - Saul, Meike J.
N1 - © 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
PY - 2024/2
Y1 - 2024/2
N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
KW - Extracellular Vesicles/metabolism
KW - Exosomes/metabolism
KW - Biological Transport
KW - Biomarkers/metabolism
KW - Phenotype
U2 - 10.1002/jev2.12404
DO - 10.1002/jev2.12404
M3 - Guideline, recommendation, statement
C2 - 38326288
VL - 13
SP - e12404
JO - J EXTRACELL VESICLES
JF - J EXTRACELL VESICLES
SN - 2001-3078
IS - 2
ER -