PortalPublikationeniBRET Screen of the ABCD1 Peroxisomal Network and Mutation-I...

iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations

Standard

iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations. / Lotz-Havla, Amelie S; Woidy, Mathias; Guder, Philipp; Friedel, Caroline C; Klingbeil, Julian M; Bulau, Ana-Maria; Schultze, Anja; Dahmen, Ilona; Noll-Puchta, Heidi; Kemp, Stephan; Erdmann, Ralf; Zimmer, Ralf; Muntau, Ania C; Gersting, Søren W.

in: J PROTEOME RES, Jahrgang 20, Nr. 9, 03.09.2021, S. 4366-4380.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lotz-Havla, AS, Woidy, M, Guder, P, Friedel, CC, Klingbeil, JM, Bulau, A-M, Schultze, A, Dahmen, I, Noll-Puchta, H, Kemp, S, Erdmann, R, Zimmer, R, Muntau, AC & Gersting, SW 2021, 'iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations', J PROTEOME RES, Jg. 20, Nr. 9, S. 4366-4380. https://doi.org/10.1021/acs.jproteome.1c00330

APA

Lotz-Havla, A. S., Woidy, M., Guder, P., Friedel, C. C., Klingbeil, J. M., Bulau, A-M., Schultze, A., Dahmen, I., Noll-Puchta, H., Kemp, S., Erdmann, R., Zimmer, R., Muntau, A. C., & Gersting, S. W. (2021). iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations. J PROTEOME RES, 20(9), 4366-4380. https://doi.org/10.1021/acs.jproteome.1c00330

Vancouver

Lotz-Havla AS, Woidy M, Guder P, Friedel CC, Klingbeil JM, Bulau A-M et al. iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations. J PROTEOME RES. 2021 Sep 3;20(9):4366-4380. https://doi.org/10.1021/acs.jproteome.1c00330

Bibtex

@article{511e079eae1a4e6585c154dc062ea8b4,
title = "iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations",
abstract = "Mapping the network of proteins provides a powerful means to investigate the function of disease genes and to unravel the molecular basis of phenotypes. We present an automated informatics-aided and bioluminescence resonance energy transfer-based approach (iBRET) enabling high-confidence detection of protein-protein interactions in living mammalian cells. A screen of the ABCD1 protein, which is affected in X-linked adrenoleukodystrophy (X-ALD), against an organelle library of peroxisomal proteins demonstrated applicability of iBRET for large-scale experiments. We identified novel protein-protein interactions for ABCD1 (with ALDH3A2, DAO, ECI2, FAR1, PEX10, PEX13, PEX5, PXMP2, and PIPOX), mapped its position within the peroxisomal protein-protein interaction network, and determined that pathogenic missense variants in ABCD1 alter the interaction with selected binding partners. These findings provide mechanistic insights into pathophysiology of X-ALD and may foster the identification of new disease modifiers.",
keywords = "ATP Binding Cassette Transporter, Subfamily D, Member 1/genetics, ATP-Binding Cassette Transporters/genetics, Animals, Energy Transfer, Fatty Acids, Informatics, Mutation",
author = "Lotz-Havla, {Amelie S} and Mathias Woidy and Philipp Guder and Friedel, {Caroline C} and Klingbeil, {Julian M} and Ana-Maria Bulau and Anja Schultze and Ilona Dahmen and Heidi Noll-Puchta and Stephan Kemp and Ralf Erdmann and Ralf Zimmer and Muntau, {Ania C} and Gersting, {S{\o}ren W}",
year = "2021",
month = sep,
day = "3",
doi = "10.1021/acs.jproteome.1c00330",
language = "English",
volume = "20",
pages = "4366--4380",
journal = "J PROTEOME RES",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "9",

}

RIS

TY - JOUR

T1 - iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations

AU - Lotz-Havla, Amelie S

AU - Woidy, Mathias

AU - Guder, Philipp

AU - Friedel, Caroline C

AU - Klingbeil, Julian M

AU - Bulau, Ana-Maria

AU - Schultze, Anja

AU - Dahmen, Ilona

AU - Noll-Puchta, Heidi

AU - Kemp, Stephan

AU - Erdmann, Ralf

AU - Zimmer, Ralf

AU - Muntau, Ania C

AU - Gersting, Søren W

PY - 2021/9/3

Y1 - 2021/9/3

N2 - Mapping the network of proteins provides a powerful means to investigate the function of disease genes and to unravel the molecular basis of phenotypes. We present an automated informatics-aided and bioluminescence resonance energy transfer-based approach (iBRET) enabling high-confidence detection of protein-protein interactions in living mammalian cells. A screen of the ABCD1 protein, which is affected in X-linked adrenoleukodystrophy (X-ALD), against an organelle library of peroxisomal proteins demonstrated applicability of iBRET for large-scale experiments. We identified novel protein-protein interactions for ABCD1 (with ALDH3A2, DAO, ECI2, FAR1, PEX10, PEX13, PEX5, PXMP2, and PIPOX), mapped its position within the peroxisomal protein-protein interaction network, and determined that pathogenic missense variants in ABCD1 alter the interaction with selected binding partners. These findings provide mechanistic insights into pathophysiology of X-ALD and may foster the identification of new disease modifiers.

AB - Mapping the network of proteins provides a powerful means to investigate the function of disease genes and to unravel the molecular basis of phenotypes. We present an automated informatics-aided and bioluminescence resonance energy transfer-based approach (iBRET) enabling high-confidence detection of protein-protein interactions in living mammalian cells. A screen of the ABCD1 protein, which is affected in X-linked adrenoleukodystrophy (X-ALD), against an organelle library of peroxisomal proteins demonstrated applicability of iBRET for large-scale experiments. We identified novel protein-protein interactions for ABCD1 (with ALDH3A2, DAO, ECI2, FAR1, PEX10, PEX13, PEX5, PXMP2, and PIPOX), mapped its position within the peroxisomal protein-protein interaction network, and determined that pathogenic missense variants in ABCD1 alter the interaction with selected binding partners. These findings provide mechanistic insights into pathophysiology of X-ALD and may foster the identification of new disease modifiers.

KW - ATP Binding Cassette Transporter, Subfamily D, Member 1/genetics

KW - ATP-Binding Cassette Transporters/genetics

KW - Animals

KW - Energy Transfer

KW - Fatty Acids

KW - Informatics

KW - Mutation

U2 - 10.1021/acs.jproteome.1c00330

DO - 10.1021/acs.jproteome.1c00330

M3 - SCORING: Journal article

C2 - 34383492

VL - 20

SP - 4366

EP - 4380

JO - J PROTEOME RES

JF - J PROTEOME RES

SN - 1535-3893

IS - 9

ER -