PortalPublikationenHigh-resolution microbiota flow cytometry reveals dynamic co...

High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition

  • Jakob Zimmermann
  • Thomas Hübschmann
  • Florian Schattenberg
  • Joachim Schumann
  • Pawel Durek
  • René Riedel
  • Marie Friedrich
  • Rainer Glauben
  • Britta Siegmund
  • Andreas Radbruch
  • Susann Müller
  • Hyun-Dong Chang

Abstract

Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.

Bibliografische Daten

OriginalspracheEnglisch
ISSN0014-2980
DOIs
StatusVeröffentlicht - 05.2016
Extern publiziertJa

Anmerkungen des Dekanats

© 2016 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PubMed 26909672