High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Abstract
Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
Bibliographical data
Original language | English |
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ISSN | 0014-2980 |
DOIs | |
Publication status | Published - 05.2016 |
Externally published | Yes |
Comment Deanary
© 2016 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PubMed | 26909672 |
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