High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition

Standard

High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition. / Zimmermann, Jakob; Hübschmann, Thomas; Schattenberg, Florian; Schumann, Joachim; Durek, Pawel; Riedel, René; Friedrich, Marie; Glauben, Rainer; Siegmund, Britta; Radbruch, Andreas; Müller, Susann; Chang, Hyun-Dong.

in: EUR J IMMUNOL, Jahrgang 46, Nr. 5, 05.2016, S. 1300-3.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Zimmermann, J, Hübschmann, T, Schattenberg, F, Schumann, J, Durek, P, Riedel, R, Friedrich, M, Glauben, R, Siegmund, B, Radbruch, A, Müller, S & Chang, H-D 2016, 'High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition', EUR J IMMUNOL, Jg. 46, Nr. 5, S. 1300-3. https://doi.org/10.1002/eji.201646297

APA

Zimmermann, J., Hübschmann, T., Schattenberg, F., Schumann, J., Durek, P., Riedel, R., Friedrich, M., Glauben, R., Siegmund, B., Radbruch, A., Müller, S., & Chang, H-D. (2016). High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition. EUR J IMMUNOL, 46(5), 1300-3. https://doi.org/10.1002/eji.201646297

Vancouver

Bibtex

@article{255767424f044395895f2c08480369f5,
title = "High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition",
abstract = "Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.",
keywords = "Animals, Bacteria/classification, Colitis/microbiology, Feces/microbiology, Flow Cytometry/methods, Gastrointestinal Microbiome, Humans, Inflammatory Bowel Diseases/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics",
author = "Jakob Zimmermann and Thomas H{\"u}bschmann and Florian Schattenberg and Joachim Schumann and Pawel Durek and Ren{\'e} Riedel and Marie Friedrich and Rainer Glauben and Britta Siegmund and Andreas Radbruch and Susann M{\"u}ller and Hyun-Dong Chang",
note = "{\textcopyright} 2016 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2016",
month = may,
doi = "10.1002/eji.201646297",
language = "English",
volume = "46",
pages = "1300--3",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "5",

}

RIS

TY - JOUR

T1 - High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition

AU - Zimmermann, Jakob

AU - Hübschmann, Thomas

AU - Schattenberg, Florian

AU - Schumann, Joachim

AU - Durek, Pawel

AU - Riedel, René

AU - Friedrich, Marie

AU - Glauben, Rainer

AU - Siegmund, Britta

AU - Radbruch, Andreas

AU - Müller, Susann

AU - Chang, Hyun-Dong

N1 - © 2016 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016/5

Y1 - 2016/5

N2 - Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.

AB - Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.

KW - Animals

KW - Bacteria/classification

KW - Colitis/microbiology

KW - Feces/microbiology

KW - Flow Cytometry/methods

KW - Gastrointestinal Microbiome

KW - Humans

KW - Inflammatory Bowel Diseases/microbiology

KW - Phylogeny

KW - RNA, Ribosomal, 16S/genetics

U2 - 10.1002/eji.201646297

DO - 10.1002/eji.201646297

M3 - SCORING: Journal article

C2 - 26909672

VL - 46

SP - 1300

EP - 1303

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 5

ER -