ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.

Xiati Guli; Tursonjan Tokay; Marco Rohde; Roland Bender; Rüdiger Köhling; Timo Kirschstein

doi:10.1155/2012/237913

ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.

Standard

ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses. / Guli, Xiati; Tokay, Tursonjan; Rohde, Marco; Bender, Roland; Köhling, Rüdiger; Kirschstein, Timo.

In: NEURAL PLAST, Vol. 2012, 2012, p. 237913.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Guli, X, Tokay, T, Rohde, M, Bender, R, Köhling, R & Kirschstein, T 2012, 'ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.', NEURAL PLAST, vol. 2012, pp. 237913. https://doi.org/10.1155/2012/237913

APA

Guli, X., Tokay, T., Rohde, M., Bender, R., Köhling, R., & Kirschstein, T. (2012). ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses. NEURAL PLAST, 2012, 237913. https://doi.org/10.1155/2012/237913

Vancouver

Guli X, Tokay T, Rohde M, Bender R, Köhling R, Kirschstein T. ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses. NEURAL PLAST. 2012;2012:237913. https://doi.org/10.1155/2012/237913

Bibtex

@article{6a4b420645944e71af3cec1f26e01508,

title = "ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.",

abstract = "Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression (LTD) at the medial perforant path-granule cell synapse in early postnatal (P9-15) and adult (P30-60) rats. LTD was elicited in P9-15 slices using low-frequency stimulation (LFS, 900 pulses, 1?Hz; 80 ± 4% of baseline). Application of the specific HCN channel blocker ZD7288 (10??M) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9-15 slices (85 ± 6%), but bath application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.",

keywords = "Animals, Male, Rats, Rats, Sprague-Dawley, Electric Stimulation, Animals, Newborn, Patch-Clamp Techniques, Neurons/*physiology, Nitric Oxide Synthase/antagonists & inhibitors, Enzyme Inhibitors/pharmacology, Cardiotonic Agents/*pharmacology, Cyclic Nucleotide-Gated Cation Channels/drug effects, Cytoplasmic Granules/drug effects, Excitatory Postsynaptic Potentials/drug effects, NG-Nitroarginine Methyl Ester/pharmacology, Neuronal Plasticity/*drug effects, Perforant Pathway/*drug effects, Potassium Channels/drug effects, Pyrimidines/*pharmacology, Receptors, N-Methyl-D-Aspartate/drug effects, Synapses/*drug effects, Animals, Male, Rats, Rats, Sprague-Dawley, Electric Stimulation, Animals, Newborn, Patch-Clamp Techniques, Neurons/*physiology, Nitric Oxide Synthase/antagonists & inhibitors, Enzyme Inhibitors/pharmacology, Cardiotonic Agents/*pharmacology, Cyclic Nucleotide-Gated Cation Channels/drug effects, Cytoplasmic Granules/drug effects, Excitatory Postsynaptic Potentials/drug effects, NG-Nitroarginine Methyl Ester/pharmacology, Neuronal Plasticity/*drug effects, Perforant Pathway/*drug effects, Potassium Channels/drug effects, Pyrimidines/*pharmacology, Receptors, N-Methyl-D-Aspartate/drug effects, Synapses/*drug effects",

author = "Xiati Guli and Tursonjan Tokay and Marco Rohde and Roland Bender and R{\"u}diger K{\"o}hling and Timo Kirschstein",

year = "2012",

doi = "10.1155/2012/237913",

language = "English",

volume = "2012",

pages = "237913",

journal = "NEURAL PLAST",

issn = "2090-5904",

publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.

AU - Guli, Xiati

AU - Tokay, Tursonjan

AU - Rohde, Marco

AU - Bender, Roland

AU - Köhling, Rüdiger

AU - Kirschstein, Timo

PY - 2012

Y1 - 2012

N2 - Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression (LTD) at the medial perforant path-granule cell synapse in early postnatal (P9-15) and adult (P30-60) rats. LTD was elicited in P9-15 slices using low-frequency stimulation (LFS, 900 pulses, 1?Hz; 80 ± 4% of baseline). Application of the specific HCN channel blocker ZD7288 (10??M) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9-15 slices (85 ± 6%), but bath application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.

AB - Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression (LTD) at the medial perforant path-granule cell synapse in early postnatal (P9-15) and adult (P30-60) rats. LTD was elicited in P9-15 slices using low-frequency stimulation (LFS, 900 pulses, 1?Hz; 80 ± 4% of baseline). Application of the specific HCN channel blocker ZD7288 (10??M) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9-15 slices (85 ± 6%), but bath application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.

KW - Animals

KW - Male

KW - Rats

KW - Rats, Sprague-Dawley

KW - Electric Stimulation

KW - Animals, Newborn

KW - Patch-Clamp Techniques

KW - Neurons/physiology

KW - Nitric Oxide Synthase/antagonists & inhibitors

KW - Enzyme Inhibitors/pharmacology

KW - Cardiotonic Agents/pharmacology

KW - Cyclic Nucleotide-Gated Cation Channels/drug effects

KW - Cytoplasmic Granules/drug effects

KW - Excitatory Postsynaptic Potentials/drug effects

KW - NG-Nitroarginine Methyl Ester/pharmacology

KW - Neuronal Plasticity/drug effects

KW - Perforant Pathway/drug effects

KW - Potassium Channels/drug effects

KW - Pyrimidines/pharmacology

KW - Receptors, N-Methyl-D-Aspartate/drug effects

KW - Synapses/drug effects