ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.
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ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses. / Guli, Xiati; Tokay, Tursonjan; Rohde, Marco; Bender, Roland; Köhling, Rüdiger; Kirschstein, Timo.
in: NEURAL PLAST, Jahrgang 2012, 2012, S. 237913.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses.
AU - Guli, Xiati
AU - Tokay, Tursonjan
AU - Rohde, Marco
AU - Bender, Roland
AU - Köhling, Rüdiger
AU - Kirschstein, Timo
PY - 2012
Y1 - 2012
N2 - Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression (LTD) at the medial perforant path-granule cell synapse in early postnatal (P9-15) and adult (P30-60) rats. LTD was elicited in P9-15 slices using low-frequency stimulation (LFS, 900 pulses, 1?Hz; 80 ± 4% of baseline). Application of the specific HCN channel blocker ZD7288 (10??M) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9-15 slices (85 ± 6%), but bath application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.
AB - Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression (LTD) at the medial perforant path-granule cell synapse in early postnatal (P9-15) and adult (P30-60) rats. LTD was elicited in P9-15 slices using low-frequency stimulation (LFS, 900 pulses, 1?Hz; 80 ± 4% of baseline). Application of the specific HCN channel blocker ZD7288 (10??M) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9-15 slices (85 ± 6%), but bath application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.
KW - Animals
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Electric Stimulation
KW - Animals, Newborn
KW - Patch-Clamp Techniques
KW - Neurons/physiology
KW - Nitric Oxide Synthase/antagonists & inhibitors
KW - Enzyme Inhibitors/pharmacology
KW - Cardiotonic Agents/pharmacology
KW - Cyclic Nucleotide-Gated Cation Channels/drug effects
KW - Cytoplasmic Granules/drug effects
KW - Excitatory Postsynaptic Potentials/drug effects
KW - NG-Nitroarginine Methyl Ester/pharmacology
KW - Neuronal Plasticity/drug effects
KW - Perforant Pathway/drug effects
KW - Potassium Channels/drug effects
KW - Pyrimidines/pharmacology
KW - Receptors, N-Methyl-D-Aspartate/drug effects
KW - Synapses/drug effects
KW - Animals
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Electric Stimulation
KW - Animals, Newborn
KW - Patch-Clamp Techniques
KW - Neurons/physiology
KW - Nitric Oxide Synthase/antagonists & inhibitors
KW - Enzyme Inhibitors/pharmacology
KW - Cardiotonic Agents/pharmacology
KW - Cyclic Nucleotide-Gated Cation Channels/drug effects
KW - Cytoplasmic Granules/drug effects
KW - Excitatory Postsynaptic Potentials/drug effects
KW - NG-Nitroarginine Methyl Ester/pharmacology
KW - Neuronal Plasticity/drug effects
KW - Perforant Pathway/drug effects
KW - Potassium Channels/drug effects
KW - Pyrimidines/pharmacology
KW - Receptors, N-Methyl-D-Aspartate/drug effects
KW - Synapses/drug effects
U2 - 10.1155/2012/237913
DO - 10.1155/2012/237913
M3 - SCORING: Journal article
VL - 2012
SP - 237913
JO - NEURAL PLAST
JF - NEURAL PLAST
SN - 2090-5904
ER -