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Vesicular release probability sets the strength of individual Schaffer collateral synapses

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Vesicular release probability sets the strength of individual Schaffer collateral synapses. / Dürst, Céline D; Wiegert, J Simon; Schulze, Christian; Helassa, Nordine; Török, Katalin; Oertner, Thomas G.

In: NAT COMMUN, Vol. 13, No. 1, 6126, 17.10.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dürst, CD, Wiegert, JS, Schulze, C, Helassa, N, Török, K & Oertner, TG 2022, 'Vesicular release probability sets the strength of individual Schaffer collateral synapses', NAT COMMUN, vol. 13, no. 1, 6126. https://doi.org/10.1038/s41467-022-33565-6

APA

Dürst, C. D., Wiegert, J. S., Schulze, C., Helassa, N., Török, K., & Oertner, T. G. (2022). Vesicular release probability sets the strength of individual Schaffer collateral synapses. NAT COMMUN, 13(1), [6126]. https://doi.org/10.1038/s41467-022-33565-6

Vancouver

Dürst CD, Wiegert JS, Schulze C, Helassa N, Török K, Oertner TG. Vesicular release probability sets the strength of individual Schaffer collateral synapses. NAT COMMUN. 2022 Oct 17;13(1). 6126. https://doi.org/10.1038/s41467-022-33565-6

Bibtex

@article{746631519ab945d59dc16a09fa620560,
title = "Vesicular release probability sets the strength of individual Schaffer collateral synapses",
abstract = "Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.",
keywords = "Calcium, Glutamic Acid, Hippocampus/physiology, Neurotransmitter Agents, Presynaptic Terminals/physiology, Probability, Synapses/physiology, Synaptic Transmission/physiology, Synaptic Vesicles/physiology",
author = "D{\"u}rst, {C{\'e}line D} and Wiegert, {J Simon} and Christian Schulze and Nordine Helassa and Katalin T{\"o}r{\"o}k and Oertner, {Thomas G}",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
month = oct,
day = "17",
doi = "10.1038/s41467-022-33565-6",
language = "English",
volume = "13",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Vesicular release probability sets the strength of individual Schaffer collateral synapses

AU - Dürst, Céline D

AU - Wiegert, J Simon

AU - Schulze, Christian

AU - Helassa, Nordine

AU - Török, Katalin

AU - Oertner, Thomas G

N1 - © 2022. The Author(s).

PY - 2022/10/17

Y1 - 2022/10/17

N2 - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.

AB - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.

KW - Calcium

KW - Glutamic Acid

KW - Hippocampus/physiology

KW - Neurotransmitter Agents

KW - Presynaptic Terminals/physiology

KW - Probability

KW - Synapses/physiology

KW - Synaptic Transmission/physiology

KW - Synaptic Vesicles/physiology

U2 - 10.1038/s41467-022-33565-6

DO - 10.1038/s41467-022-33565-6

M3 - SCORING: Journal article

C2 - 36253353

VL - 13

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

M1 - 6126

ER -