Vesicular release probability sets the strength of individual Schaffer collateral synapses
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Vesicular release probability sets the strength of individual Schaffer collateral synapses. / Dürst, Céline D; Wiegert, J Simon; Schulze, Christian; Helassa, Nordine; Török, Katalin; Oertner, Thomas G.
in: NAT COMMUN, Jahrgang 13, Nr. 1, 6126, 17.10.2022.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Vesicular release probability sets the strength of individual Schaffer collateral synapses
AU - Dürst, Céline D
AU - Wiegert, J Simon
AU - Schulze, Christian
AU - Helassa, Nordine
AU - Török, Katalin
AU - Oertner, Thomas G
N1 - © 2022. The Author(s).
PY - 2022/10/17
Y1 - 2022/10/17
N2 - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.
AB - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.
KW - Calcium
KW - Glutamic Acid
KW - Hippocampus/physiology
KW - Neurotransmitter Agents
KW - Presynaptic Terminals/physiology
KW - Probability
KW - Synapses/physiology
KW - Synaptic Transmission/physiology
KW - Synaptic Vesicles/physiology
U2 - 10.1038/s41467-022-33565-6
DO - 10.1038/s41467-022-33565-6
M3 - SCORING: Journal article
C2 - 36253353
VL - 13
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
M1 - 6126
ER -