Research ExplorerPublicationsValidation and Application of an HPLC-UV Method for Routine ...

Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol

Standard

Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol. / Zimmer, Julia; Röhr, Anka C; Kluge, Stefan; Faller, Jonas; Frey, Otto R; Wichmann, Dominic; König, Christina.

In: ANTIBIOTICS-BASEL, Vol. 10, No. 3, 28.02.2021, p. 242.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Zimmer, J, Röhr, AC, Kluge, S, Faller, J, Frey, OR, Wichmann, D & König, C 2021, 'Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol', ANTIBIOTICS-BASEL, vol. 10, no. 3, pp. 242. https://doi.org/10.3390/antibiotics10030242

APA

Zimmer, J., Röhr, A. C., Kluge, S., Faller, J., Frey, O. R., Wichmann, D., & König, C. (2021). Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol. ANTIBIOTICS-BASEL, 10(3), 242. https://doi.org/10.3390/antibiotics10030242

Vancouver

Zimmer J, Röhr AC, Kluge S, Faller J, Frey OR, Wichmann D et al. Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol. ANTIBIOTICS-BASEL. 2021 Feb 28;10(3):242. https://doi.org/10.3390/antibiotics10030242

Bibtex

@article{629e05cf4f07453eb3b9d180668fa176,
title = "Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol",
abstract = "Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy.",
author = "Julia Zimmer and R{\"o}hr, {Anka C} and Stefan Kluge and Jonas Faller and Frey, {Otto R} and Dominic Wichmann and Christina K{\"o}nig",
year = "2021",
month = feb,
day = "28",
doi = "10.3390/antibiotics10030242",
language = "English",
volume = "10",
pages = "242",
journal = "ANTIBIOTICS-BASEL",
issn = "2079-6382",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
number = "3",

}

RIS

TY - JOUR

T1 - Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol

AU - Zimmer, Julia

AU - Röhr, Anka C

AU - Kluge, Stefan

AU - Faller, Jonas

AU - Frey, Otto R

AU - Wichmann, Dominic

AU - König, Christina

PY - 2021/2/28

Y1 - 2021/2/28

N2 - Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy.

AB - Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy.

U2 - 10.3390/antibiotics10030242

DO - 10.3390/antibiotics10030242

M3 - SCORING: Journal article

C2 - 33670891

VL - 10

SP - 242

JO - ANTIBIOTICS-BASEL

JF - ANTIBIOTICS-BASEL

SN - 2079-6382

IS - 3

ER -