Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol
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Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol. / Zimmer, Julia; Röhr, Anka C; Kluge, Stefan; Faller, Jonas; Frey, Otto R; Wichmann, Dominic; König, Christina.
in: ANTIBIOTICS-BASEL, Jahrgang 10, Nr. 3, 28.02.2021, S. 242.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol
AU - Zimmer, Julia
AU - Röhr, Anka C
AU - Kluge, Stefan
AU - Faller, Jonas
AU - Frey, Otto R
AU - Wichmann, Dominic
AU - König, Christina
PY - 2021/2/28
Y1 - 2021/2/28
N2 - Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy.
AB - Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy.
U2 - 10.3390/antibiotics10030242
DO - 10.3390/antibiotics10030242
M3 - SCORING: Journal article
C2 - 33670891
VL - 10
SP - 242
JO - ANTIBIOTICS-BASEL
JF - ANTIBIOTICS-BASEL
SN - 2079-6382
IS - 3
ER -