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Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases

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Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases. / Salamon, Johannes; Peldschus, Kersten.

In: Methods Mol Biol, Vol. 1070, 01.01.2014, p. 203-11.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Salamon, J & Peldschus, K 2014, 'Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases', Methods Mol Biol, vol. 1070, pp. 203-11. https://doi.org/10.1007/978-1-4614-8244-4_15

APA

Salamon, J., & Peldschus, K. (2014). Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases. Methods Mol Biol, 1070, 203-11. https://doi.org/10.1007/978-1-4614-8244-4_15

Vancouver

Salamon J, Peldschus K. Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases. Methods Mol Biol. 2014 Jan 1;1070:203-11. https://doi.org/10.1007/978-1-4614-8244-4_15

Bibtex

@article{6ad595ef2fdc44c29cc0630ee515f9e6,
title = "Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases",
abstract = "Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.",
keywords = "Animals, Drug Administration Routes, Magnetic Resonance Imaging, Mesenchymal Stromal Cells, Mice, Mice, Inbred BALB C, Mice, SCID, Myocardium, Neoplasm Metastasis, Single-Cell Analysis, Staining and Labeling, Ultrasonics",
author = "Johannes Salamon and Kersten Peldschus",
year = "2014",
month = jan,
day = "1",
doi = "10.1007/978-1-4614-8244-4_15",
language = "English",
volume = "1070",
pages = "203--11",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases

AU - Salamon, Johannes

AU - Peldschus, Kersten

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.

AB - Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.

KW - Animals

KW - Drug Administration Routes

KW - Magnetic Resonance Imaging

KW - Mesenchymal Stromal Cells

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, SCID

KW - Myocardium

KW - Neoplasm Metastasis

KW - Single-Cell Analysis

KW - Staining and Labeling

KW - Ultrasonics

U2 - 10.1007/978-1-4614-8244-4_15

DO - 10.1007/978-1-4614-8244-4_15

M3 - SCORING: Journal article

C2 - 24092442

VL - 1070

SP - 203

EP - 211

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -