Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases
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Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases. / Salamon, Johannes; Peldschus, Kersten.
in: Methods Mol Biol, Jahrgang 1070, 01.01.2014, S. 203-11.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Ultrasound-guided intracardial injection and in vivo magnetic resonance imaging of single cells in mice as a paradigm for hematogenous metastases
AU - Salamon, Johannes
AU - Peldschus, Kersten
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.
AB - Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.
KW - Animals
KW - Drug Administration Routes
KW - Magnetic Resonance Imaging
KW - Mesenchymal Stromal Cells
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, SCID
KW - Myocardium
KW - Neoplasm Metastasis
KW - Single-Cell Analysis
KW - Staining and Labeling
KW - Ultrasonics
U2 - 10.1007/978-1-4614-8244-4_15
DO - 10.1007/978-1-4614-8244-4_15
M3 - SCORING: Journal article
C2 - 24092442
VL - 1070
SP - 203
EP - 211
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -