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Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues

Standard

Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues. / Crosbie, Jeffrey C; Anderson, Robin L; Rothkamm, Kai; Restall, Christina M; Cann, Leonie; Ruwanpura, Saleela; Meachem, Sarah; Yagi, Naoto; Svalbe, Imants; Lewis, Robert A; Williams, Bryan R G; Rogers, Peter A W.

In: INT J RADIAT ONCOL, Vol. 77, No. 3, 01.07.2010, p. 886-94.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Crosbie, JC, Anderson, RL, Rothkamm, K, Restall, CM, Cann, L, Ruwanpura, S, Meachem, S, Yagi, N, Svalbe, I, Lewis, RA, Williams, BRG & Rogers, PAW 2010, 'Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues', INT J RADIAT ONCOL, vol. 77, no. 3, pp. 886-94. https://doi.org/10.1016/j.ijrobp.2010.01.035

APA

Crosbie, J. C., Anderson, R. L., Rothkamm, K., Restall, C. M., Cann, L., Ruwanpura, S., Meachem, S., Yagi, N., Svalbe, I., Lewis, R. A., Williams, B. R. G., & Rogers, P. A. W. (2010). Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues. INT J RADIAT ONCOL, 77(3), 886-94. https://doi.org/10.1016/j.ijrobp.2010.01.035

Vancouver

Crosbie JC, Anderson RL, Rothkamm K, Restall CM, Cann L, Ruwanpura S et al. Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues. INT J RADIAT ONCOL. 2010 Jul 1;77(3):886-94. https://doi.org/10.1016/j.ijrobp.2010.01.035

Bibtex

@article{4cb1f19632da40aea6680cabeeb9c77b,
title = "Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues",
abstract = "PURPOSE: High-dose synchrotron microbeam radiation therapy (MRT) can be effective at destroying tumors in animal models while causing very little damage to normal tissues. The aim of this study was to investigate the cellular processes behind this observation of potential clinical importance.METHODS AND MATERIALS: MRT was performed using a lattice of 25 mum-wide, planar, polychromatic, kilovoltage X-ray microbeams, with 200-microm peak separation. Inoculated EMT-6.5 tumor and normal mouse skin tissues were harvested at defined intervals post-MRT. Immunohistochemical detection of gamma-H2AX allowed precise localization of irradiated cells, which were also assessed for proliferation and apoptosis.RESULTS: MRT significantly reduced tumor cell proliferation by 24 h post-irradiation (p = 0.002). An unexpected finding was that within 24 h of MRT, peak and valley irradiated zones were indistinguishable in tumors because of extensive cell migration between the zones. This was not seen in MRT-treated normal skin, which appeared to undergo a coordinated repair response. MRT elicited an increase in median survival times of EMT-6.5 and 67NR tumor-inoculated mice similar to that achieved with conventional radiotherapy, while causing markedly less normal tissue damage.CONCLUSIONS: This study provides evidence of a differential response at a cellular level between normal and tumor tissues after synchrotron MRT.",
keywords = "Animals, Apoptosis/physiology, Biomarkers/analysis, Cell Movement/physiology, Cell Proliferation/radiation effects, DNA Breaks, Double-Stranded, DNA Repair/physiology, Female, Histones/analysis, Mammary Neoplasms, Experimental/genetics, Mice, Mice, Inbred BALB C, Radiation Injuries, Experimental/metabolism, Radiation Tolerance/genetics, Random Allocation, Skin/cytology, Synchrotrons, Time Factors",
author = "Crosbie, {Jeffrey C} and Anderson, {Robin L} and Kai Rothkamm and Restall, {Christina M} and Leonie Cann and Saleela Ruwanpura and Sarah Meachem and Naoto Yagi and Imants Svalbe and Lewis, {Robert A} and Williams, {Bryan R G} and Rogers, {Peter A W}",
note = "(c) 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = jul,
day = "1",
doi = "10.1016/j.ijrobp.2010.01.035",
language = "English",
volume = "77",
pages = "886--94",
journal = "INT J RADIAT ONCOL",
issn = "0360-3016",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Tumor cell response to synchrotron microbeam radiation therapy differs markedly from cells in normal tissues

AU - Crosbie, Jeffrey C

AU - Anderson, Robin L

AU - Rothkamm, Kai

AU - Restall, Christina M

AU - Cann, Leonie

AU - Ruwanpura, Saleela

AU - Meachem, Sarah

AU - Yagi, Naoto

AU - Svalbe, Imants

AU - Lewis, Robert A

AU - Williams, Bryan R G

AU - Rogers, Peter A W

N1 - (c) 2010 Elsevier Inc. All rights reserved.

PY - 2010/7/1

Y1 - 2010/7/1

N2 - PURPOSE: High-dose synchrotron microbeam radiation therapy (MRT) can be effective at destroying tumors in animal models while causing very little damage to normal tissues. The aim of this study was to investigate the cellular processes behind this observation of potential clinical importance.METHODS AND MATERIALS: MRT was performed using a lattice of 25 mum-wide, planar, polychromatic, kilovoltage X-ray microbeams, with 200-microm peak separation. Inoculated EMT-6.5 tumor and normal mouse skin tissues were harvested at defined intervals post-MRT. Immunohistochemical detection of gamma-H2AX allowed precise localization of irradiated cells, which were also assessed for proliferation and apoptosis.RESULTS: MRT significantly reduced tumor cell proliferation by 24 h post-irradiation (p = 0.002). An unexpected finding was that within 24 h of MRT, peak and valley irradiated zones were indistinguishable in tumors because of extensive cell migration between the zones. This was not seen in MRT-treated normal skin, which appeared to undergo a coordinated repair response. MRT elicited an increase in median survival times of EMT-6.5 and 67NR tumor-inoculated mice similar to that achieved with conventional radiotherapy, while causing markedly less normal tissue damage.CONCLUSIONS: This study provides evidence of a differential response at a cellular level between normal and tumor tissues after synchrotron MRT.

AB - PURPOSE: High-dose synchrotron microbeam radiation therapy (MRT) can be effective at destroying tumors in animal models while causing very little damage to normal tissues. The aim of this study was to investigate the cellular processes behind this observation of potential clinical importance.METHODS AND MATERIALS: MRT was performed using a lattice of 25 mum-wide, planar, polychromatic, kilovoltage X-ray microbeams, with 200-microm peak separation. Inoculated EMT-6.5 tumor and normal mouse skin tissues were harvested at defined intervals post-MRT. Immunohistochemical detection of gamma-H2AX allowed precise localization of irradiated cells, which were also assessed for proliferation and apoptosis.RESULTS: MRT significantly reduced tumor cell proliferation by 24 h post-irradiation (p = 0.002). An unexpected finding was that within 24 h of MRT, peak and valley irradiated zones were indistinguishable in tumors because of extensive cell migration between the zones. This was not seen in MRT-treated normal skin, which appeared to undergo a coordinated repair response. MRT elicited an increase in median survival times of EMT-6.5 and 67NR tumor-inoculated mice similar to that achieved with conventional radiotherapy, while causing markedly less normal tissue damage.CONCLUSIONS: This study provides evidence of a differential response at a cellular level between normal and tumor tissues after synchrotron MRT.

KW - Animals

KW - Apoptosis/physiology

KW - Biomarkers/analysis

KW - Cell Movement/physiology

KW - Cell Proliferation/radiation effects

KW - DNA Breaks, Double-Stranded

KW - DNA Repair/physiology

KW - Female

KW - Histones/analysis

KW - Mammary Neoplasms, Experimental/genetics

KW - Mice

KW - Mice, Inbred BALB C

KW - Radiation Injuries, Experimental/metabolism

KW - Radiation Tolerance/genetics

KW - Random Allocation

KW - Skin/cytology

KW - Synchrotrons

KW - Time Factors

U2 - 10.1016/j.ijrobp.2010.01.035

DO - 10.1016/j.ijrobp.2010.01.035

M3 - SCORING: Journal article

C2 - 20510199

VL - 77

SP - 886

EP - 894

JO - INT J RADIAT ONCOL

JF - INT J RADIAT ONCOL

SN - 0360-3016

IS - 3

ER -