Tracheal replacement in rabbits with a new composite silicone-metallic prosthesis

TY - JOUR

T1 - Tracheal replacement in rabbits with a new composite silicone-metallic prosthesis

AU - Dodge-Khatami, Ali

AU - Niessen, Hans W M

AU - Koole, Leo H

AU - Klein, Marloes G

AU - van Gulik, Thomas M

AU - de Mol, Bas A J M

PY - 2003/9

Y1 - 2003/9

N2 - A new composite silicone-metallic prosthesis was tested, studying the potential for respiratory epithelial covering over the biocompatible inner lining, in a rabbit survival model. Seven New Zealand White rabbits underwent near-total excision of their trachea and implantation of a sterile prosthesis. After 2 months, they were sacrificed and the prostheses were retrieved. Specimens were fixed and histologically examined for tissue reaction around the prosthesis, at the anastomotic lines, and particularly for the presence or absence of epithelialization of the inner lumen over the biocompatible surface. All rabbits survived the operation. At 2 months, the outer layer of the prosthesis was consistently covered with fibrosis and neutrophils. The inner layer showed necrotic cells and scant re-epithelialization over the biocompatible lining, up to 5 mm beyond the anastomosis, with no evidence of organized respiratory epithelium in the middle sections. The new prosthesis is a viable temporary solution for airway replacement in rabbits. Granulation tissue was not observed at the anastomosis, and re-epithelialization did occur, but failed to achieve full-length luminal covering. The potential for granulation tissue does not yet make this an ideal long-term solution. Improvements in prosthesis design or biocompatibility are required, and need to be re-evaluated before applicability for chronic use.

AB - A new composite silicone-metallic prosthesis was tested, studying the potential for respiratory epithelial covering over the biocompatible inner lining, in a rabbit survival model. Seven New Zealand White rabbits underwent near-total excision of their trachea and implantation of a sterile prosthesis. After 2 months, they were sacrificed and the prostheses were retrieved. Specimens were fixed and histologically examined for tissue reaction around the prosthesis, at the anastomotic lines, and particularly for the presence or absence of epithelialization of the inner lumen over the biocompatible surface. All rabbits survived the operation. At 2 months, the outer layer of the prosthesis was consistently covered with fibrosis and neutrophils. The inner layer showed necrotic cells and scant re-epithelialization over the biocompatible lining, up to 5 mm beyond the anastomosis, with no evidence of organized respiratory epithelium in the middle sections. The new prosthesis is a viable temporary solution for airway replacement in rabbits. Granulation tissue was not observed at the anastomosis, and re-epithelialization did occur, but failed to achieve full-length luminal covering. The potential for granulation tissue does not yet make this an ideal long-term solution. Improvements in prosthesis design or biocompatibility are required, and need to be re-evaluated before applicability for chronic use.

KW - Animals

KW - Biocompatible Materials/therapeutic use

KW - Metals/therapeutic use

KW - Prostheses and Implants

KW - Prosthesis Implantation/instrumentation

KW - Rabbits

KW - Silicones/therapeutic use

KW - Trachea/surgery

U2 - 10.1177/021849230301100314

DO - 10.1177/021849230301100314

M3 - SCORING: Journal article

C2 - 14514557

VL - 11

SP - 245

EP - 249

JO - ASIAN CARD THORAC AN

JF - ASIAN CARD THORAC AN

SN - 0218-4923

IS - 3

ER -