Toxicity study of erucylphosphocholine in a rat model
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Toxicity study of erucylphosphocholine in a rat model. / Schuettauf, Frank; Eibl, Kirsten H; Thaler, Sebastian; Shinoda, Kei; Rejdak, Robert; May, C Albrecht; Blatsios, Georgios; Welge-Lussen, Ulrich.
In: CURR EYE RES, Vol. 30, No. 9, 09.2005, p. 813-20.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Toxicity study of erucylphosphocholine in a rat model
AU - Schuettauf, Frank
AU - Eibl, Kirsten H
AU - Thaler, Sebastian
AU - Shinoda, Kei
AU - Rejdak, Robert
AU - May, C Albrecht
AU - Blatsios, Georgios
AU - Welge-Lussen, Ulrich
PY - 2005/9
Y1 - 2005/9
N2 - PURPOSE: To investigate the effect of intraocular erucylphosphocholine (ErPC) on the retina, the retinal pigment epithelium (RPE), and the choroid in an in vivo rat model.METHODS: Adult male Brown Norway rats were injected intravitreally with ErPC dissolved in balanced salt solution (BSS) at a final concentration of 10 or 100 microM with BSS serving as control. Adverse effects on the anterior and posterior segment were assessed by slit-lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by electroretinography (ERG), retinal ganglion cell (RGC) quantification, and histology 7 days after intravitreal administration of ErPC.RESULTS: There was neither a statistically significant difference in the clinical examination nor in the ERG waves of treated versus control rats 7 days after intravitreal administration of ErPC. Correspondingly, the number of RGC after BSS injection did not differ significantly from ErPC-injected animals. Histologic sections of the posterior segment of 10 and 100 microM ErPC-injected rats did not show any signs of retinal toxicity. Electron microscopy did not display a difference between the 10 microM and the control group. Only the 100 microM-injected animals showed a discrete irregularity of the Müller cell and the retinal ganglion cell cytoplasm at the ultrastructural level.CONCLUSIONS: ErPC can safely be injected into the vitreous of adult rats at a concentration of 10 microM without any retinal toxicity. Even a 10-fold increase in ErPC concentration leads only to a discrete cytoplasmic irregularity of the innermost retinal layers.
AB - PURPOSE: To investigate the effect of intraocular erucylphosphocholine (ErPC) on the retina, the retinal pigment epithelium (RPE), and the choroid in an in vivo rat model.METHODS: Adult male Brown Norway rats were injected intravitreally with ErPC dissolved in balanced salt solution (BSS) at a final concentration of 10 or 100 microM with BSS serving as control. Adverse effects on the anterior and posterior segment were assessed by slit-lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by electroretinography (ERG), retinal ganglion cell (RGC) quantification, and histology 7 days after intravitreal administration of ErPC.RESULTS: There was neither a statistically significant difference in the clinical examination nor in the ERG waves of treated versus control rats 7 days after intravitreal administration of ErPC. Correspondingly, the number of RGC after BSS injection did not differ significantly from ErPC-injected animals. Histologic sections of the posterior segment of 10 and 100 microM ErPC-injected rats did not show any signs of retinal toxicity. Electron microscopy did not display a difference between the 10 microM and the control group. Only the 100 microM-injected animals showed a discrete irregularity of the Müller cell and the retinal ganglion cell cytoplasm at the ultrastructural level.CONCLUSIONS: ErPC can safely be injected into the vitreous of adult rats at a concentration of 10 microM without any retinal toxicity. Even a 10-fold increase in ErPC concentration leads only to a discrete cytoplasmic irregularity of the innermost retinal layers.
KW - Animals
KW - Cell Count
KW - Choroid/drug effects
KW - Electroretinography/drug effects
KW - Injections
KW - Male
KW - Phosphorylcholine/analogs & derivatives
KW - Pigment Epithelium of Eye/drug effects
KW - Rats
KW - Rats, Inbred BN
KW - Retina/drug effects
KW - Vitreous Body
U2 - 10.1080/02713680591006093
DO - 10.1080/02713680591006093
M3 - SCORING: Journal article
C2 - 16146926
VL - 30
SP - 813
EP - 820
JO - CURR EYE RES
JF - CURR EYE RES
SN - 0271-3683
IS - 9
ER -