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Toxicity study of erucylphosphocholine in a rat model

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Toxicity study of erucylphosphocholine in a rat model. / Schuettauf, Frank; Eibl, Kirsten H; Thaler, Sebastian; Shinoda, Kei; Rejdak, Robert; May, C Albrecht; Blatsios, Georgios; Welge-Lussen, Ulrich.

in: CURR EYE RES, Jahrgang 30, Nr. 9, 09.2005, S. 813-20.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schuettauf, F, Eibl, KH, Thaler, S, Shinoda, K, Rejdak, R, May, CA, Blatsios, G & Welge-Lussen, U 2005, 'Toxicity study of erucylphosphocholine in a rat model', CURR EYE RES, Jg. 30, Nr. 9, S. 813-20. https://doi.org/10.1080/02713680591006093

APA

Schuettauf, F., Eibl, K. H., Thaler, S., Shinoda, K., Rejdak, R., May, C. A., Blatsios, G., & Welge-Lussen, U. (2005). Toxicity study of erucylphosphocholine in a rat model. CURR EYE RES, 30(9), 813-20. https://doi.org/10.1080/02713680591006093

Vancouver

Schuettauf F, Eibl KH, Thaler S, Shinoda K, Rejdak R, May CA et al. Toxicity study of erucylphosphocholine in a rat model. CURR EYE RES. 2005 Sep;30(9):813-20. https://doi.org/10.1080/02713680591006093

Bibtex

@article{93cef5b558324e51bf17ae34971b6e2c,
title = "Toxicity study of erucylphosphocholine in a rat model",
abstract = "PURPOSE: To investigate the effect of intraocular erucylphosphocholine (ErPC) on the retina, the retinal pigment epithelium (RPE), and the choroid in an in vivo rat model.METHODS: Adult male Brown Norway rats were injected intravitreally with ErPC dissolved in balanced salt solution (BSS) at a final concentration of 10 or 100 microM with BSS serving as control. Adverse effects on the anterior and posterior segment were assessed by slit-lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by electroretinography (ERG), retinal ganglion cell (RGC) quantification, and histology 7 days after intravitreal administration of ErPC.RESULTS: There was neither a statistically significant difference in the clinical examination nor in the ERG waves of treated versus control rats 7 days after intravitreal administration of ErPC. Correspondingly, the number of RGC after BSS injection did not differ significantly from ErPC-injected animals. Histologic sections of the posterior segment of 10 and 100 microM ErPC-injected rats did not show any signs of retinal toxicity. Electron microscopy did not display a difference between the 10 microM and the control group. Only the 100 microM-injected animals showed a discrete irregularity of the M{\"u}ller cell and the retinal ganglion cell cytoplasm at the ultrastructural level.CONCLUSIONS: ErPC can safely be injected into the vitreous of adult rats at a concentration of 10 microM without any retinal toxicity. Even a 10-fold increase in ErPC concentration leads only to a discrete cytoplasmic irregularity of the innermost retinal layers.",
keywords = "Animals, Cell Count, Choroid/drug effects, Electroretinography/drug effects, Injections, Male, Phosphorylcholine/analogs & derivatives, Pigment Epithelium of Eye/drug effects, Rats, Rats, Inbred BN, Retina/drug effects, Vitreous Body",
author = "Frank Schuettauf and Eibl, {Kirsten H} and Sebastian Thaler and Kei Shinoda and Robert Rejdak and May, {C Albrecht} and Georgios Blatsios and Ulrich Welge-Lussen",
year = "2005",
month = sep,
doi = "10.1080/02713680591006093",
language = "English",
volume = "30",
pages = "813--20",
journal = "CURR EYE RES",
issn = "0271-3683",
publisher = "Taylor & Francis",
number = "9",

}

RIS

TY - JOUR

T1 - Toxicity study of erucylphosphocholine in a rat model

AU - Schuettauf, Frank

AU - Eibl, Kirsten H

AU - Thaler, Sebastian

AU - Shinoda, Kei

AU - Rejdak, Robert

AU - May, C Albrecht

AU - Blatsios, Georgios

AU - Welge-Lussen, Ulrich

PY - 2005/9

Y1 - 2005/9

N2 - PURPOSE: To investigate the effect of intraocular erucylphosphocholine (ErPC) on the retina, the retinal pigment epithelium (RPE), and the choroid in an in vivo rat model.METHODS: Adult male Brown Norway rats were injected intravitreally with ErPC dissolved in balanced salt solution (BSS) at a final concentration of 10 or 100 microM with BSS serving as control. Adverse effects on the anterior and posterior segment were assessed by slit-lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by electroretinography (ERG), retinal ganglion cell (RGC) quantification, and histology 7 days after intravitreal administration of ErPC.RESULTS: There was neither a statistically significant difference in the clinical examination nor in the ERG waves of treated versus control rats 7 days after intravitreal administration of ErPC. Correspondingly, the number of RGC after BSS injection did not differ significantly from ErPC-injected animals. Histologic sections of the posterior segment of 10 and 100 microM ErPC-injected rats did not show any signs of retinal toxicity. Electron microscopy did not display a difference between the 10 microM and the control group. Only the 100 microM-injected animals showed a discrete irregularity of the Müller cell and the retinal ganglion cell cytoplasm at the ultrastructural level.CONCLUSIONS: ErPC can safely be injected into the vitreous of adult rats at a concentration of 10 microM without any retinal toxicity. Even a 10-fold increase in ErPC concentration leads only to a discrete cytoplasmic irregularity of the innermost retinal layers.

AB - PURPOSE: To investigate the effect of intraocular erucylphosphocholine (ErPC) on the retina, the retinal pigment epithelium (RPE), and the choroid in an in vivo rat model.METHODS: Adult male Brown Norway rats were injected intravitreally with ErPC dissolved in balanced salt solution (BSS) at a final concentration of 10 or 100 microM with BSS serving as control. Adverse effects on the anterior and posterior segment were assessed by slit-lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by electroretinography (ERG), retinal ganglion cell (RGC) quantification, and histology 7 days after intravitreal administration of ErPC.RESULTS: There was neither a statistically significant difference in the clinical examination nor in the ERG waves of treated versus control rats 7 days after intravitreal administration of ErPC. Correspondingly, the number of RGC after BSS injection did not differ significantly from ErPC-injected animals. Histologic sections of the posterior segment of 10 and 100 microM ErPC-injected rats did not show any signs of retinal toxicity. Electron microscopy did not display a difference between the 10 microM and the control group. Only the 100 microM-injected animals showed a discrete irregularity of the Müller cell and the retinal ganglion cell cytoplasm at the ultrastructural level.CONCLUSIONS: ErPC can safely be injected into the vitreous of adult rats at a concentration of 10 microM without any retinal toxicity. Even a 10-fold increase in ErPC concentration leads only to a discrete cytoplasmic irregularity of the innermost retinal layers.

KW - Animals

KW - Cell Count

KW - Choroid/drug effects

KW - Electroretinography/drug effects

KW - Injections

KW - Male

KW - Phosphorylcholine/analogs & derivatives

KW - Pigment Epithelium of Eye/drug effects

KW - Rats

KW - Rats, Inbred BN

KW - Retina/drug effects

KW - Vitreous Body

U2 - 10.1080/02713680591006093

DO - 10.1080/02713680591006093

M3 - SCORING: Journal article

C2 - 16146926

VL - 30

SP - 813

EP - 820

JO - CURR EYE RES

JF - CURR EYE RES

SN - 0271-3683

IS - 9

ER -