Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations
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Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations : generating neurons from human skin? / Kruse, Charli; Bodó, Enikö; Petschnik, Anna E; Danner, Sandra; Tiede, Stephan; Paus, Ralf.
In: EXP DERMATOL, Vol. 15, No. 10, 10.2006, p. 794-800.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations
T2 - generating neurons from human skin?
AU - Kruse, Charli
AU - Bodó, Enikö
AU - Petschnik, Anna E
AU - Danner, Sandra
AU - Tiede, Stephan
AU - Paus, Ralf
PY - 2006/10
Y1 - 2006/10
N2 - Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.
AB - Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.
KW - Cell Differentiation
KW - Cell Separation
KW - Cells, Cultured
KW - Humans
KW - Intermediate Filament Proteins
KW - Nerve Tissue Proteins
KW - Nestin
KW - Neuroglia
KW - Neurons
KW - Scalp
KW - Skin
KW - Stem Cells
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1600-0625.2006.00471.x
DO - 10.1111/j.1600-0625.2006.00471.x
M3 - SCORING: Journal article
C2 - 16984261
VL - 15
SP - 794
EP - 800
JO - EXP DERMATOL
JF - EXP DERMATOL
SN - 0906-6705
IS - 10
ER -