Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin?

Charli Kruse; Enikö Bodó; Anna E Petschnik; Sandra Danner; Stephan Tiede; Ralf Paus

doi:10.1111/j.1600-0625.2006.00471.x

Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations

Standard

Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations : generating neurons from human skin? / Kruse, Charli; Bodó, Enikö; Petschnik, Anna E; Danner, Sandra; Tiede, Stephan; Paus, Ralf.

in: EXP DERMATOL, Jahrgang 15, Nr. 10, 10.2006, S. 794-800.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kruse, C, Bodó, E, Petschnik, AE, Danner, S, Tiede, S & Paus, R 2006, 'Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin?', EXP DERMATOL, Jg. 15, Nr. 10, S. 794-800. https://doi.org/10.1111/j.1600-0625.2006.00471.x

APA

Kruse, C., Bodó, E., Petschnik, A. E., Danner, S., Tiede, S., & Paus, R. (2006). Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin? EXP DERMATOL, 15(10), 794-800. https://doi.org/10.1111/j.1600-0625.2006.00471.x

Vancouver

Kruse C, Bodó E, Petschnik AE, Danner S, Tiede S, Paus R. Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin? EXP DERMATOL. 2006 Okt;15(10):794-800. https://doi.org/10.1111/j.1600-0625.2006.00471.x

Bibtex

@article{254cbd39594b467a98320df8516a8feb,

title = "Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin?",

abstract = "Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.",

keywords = "Cell Differentiation, Cell Separation, Cells, Cultured, Humans, Intermediate Filament Proteins, Nerve Tissue Proteins, Nestin, Neuroglia, Neurons, Scalp, Skin, Stem Cells, Journal Article, Research Support, Non-U.S. Gov't",

author = "Charli Kruse and Enik{\"o} Bod{\'o} and Petschnik, {Anna E} and Sandra Danner and Stephan Tiede and Ralf Paus",

year = "2006",

month = oct,

doi = "10.1111/j.1600-0625.2006.00471.x",

language = "English",

volume = "15",

pages = "794--800",

journal = "EXP DERMATOL",

issn = "0906-6705",

publisher = "Wiley-Blackwell",

number = "10",

}

RIS

TY - JOUR

T1 - Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations

T2 - generating neurons from human skin?

AU - Kruse, Charli

AU - Bodó, Enikö

AU - Petschnik, Anna E

AU - Danner, Sandra

AU - Tiede, Stephan

AU - Paus, Ralf

PY - 2006/10

Y1 - 2006/10

N2 - Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.

AB - Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.

KW - Cell Differentiation

KW - Cell Separation

KW - Cells, Cultured

KW - Humans

KW - Intermediate Filament Proteins

KW - Nerve Tissue Proteins

KW - Nestin

KW - Neuroglia

KW - Neurons

KW - Scalp

KW - Skin

KW - Stem Cells

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1600-0625.2006.00471.x

DO - 10.1111/j.1600-0625.2006.00471.x

M3 - SCORING: Journal article

C2 - 16984261

VL - 15

SP - 794

EP - 800

JO - EXP DERMATOL

JF - EXP DERMATOL

SN - 0906-6705

IS - 10

ER -