Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk

TY - JOUR

T1 - Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk

AU - Molinnus, Denise

AU - Drinic, Aleksander

AU - Iken, Heiko

AU - Kröger, Nadja

AU - Zinser, Max

AU - Smeets, Ralf

AU - Köpf, Marius

AU - Kopp, Alexander

AU - Schöning, Michael J

N1 - Copyright © 2021 Elsevier B.V. All rights reserved.

PY - 2021/7/1

Y1 - 2021/7/1

N2 - In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. The flexible glucose biosensor is encapsulated by a biocompatible silicon rubber which is certificated for a safe use onto human skin. Characterization of the sensor set-up is exemplarily demonstrated by glucose measurements in buffer and Ringer's solution, while the stability of the quasi-reference electrode has been investigated versus a commercial Ag/AgCl reference electrode. Repeated bending studies validated the mechanical properties of the electrode structures. The cross-sensitivity of the biosensor against ascorbic acid, noradrenaline and adrenaline was investigated, too. Additionally, biocompatibility and degradation tests of the silk fibroin with and without thin-film platinum electrodes were carried out.

AB - In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. The flexible glucose biosensor is encapsulated by a biocompatible silicon rubber which is certificated for a safe use onto human skin. Characterization of the sensor set-up is exemplarily demonstrated by glucose measurements in buffer and Ringer's solution, while the stability of the quasi-reference electrode has been investigated versus a commercial Ag/AgCl reference electrode. Repeated bending studies validated the mechanical properties of the electrode structures. The cross-sensitivity of the biosensor against ascorbic acid, noradrenaline and adrenaline was investigated, too. Additionally, biocompatibility and degradation tests of the silk fibroin with and without thin-film platinum electrodes were carried out.

KW - Animals

KW - Biocompatible Materials

KW - Biosensing Techniques

KW - Bombyx

KW - Fibroins

KW - Humans

KW - Polymers

KW - Silk

U2 - 10.1016/j.bios.2021.113204

DO - 10.1016/j.bios.2021.113204

M3 - SCORING: Journal article

C2 - 33836429

VL - 183

JO - BIOSENS BIOELECTRON

JF - BIOSENS BIOELECTRON

SN - 0956-5663

M1 - 113204

ER -