Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk
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Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk. / Molinnus, Denise; Drinic, Aleksander; Iken, Heiko; Kröger, Nadja; Zinser, Max; Smeets, Ralf; Köpf, Marius; Kopp, Alexander; Schöning, Michael J.
in: BIOSENS BIOELECTRON, Jahrgang 183, 113204, 01.07.2021.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk
AU - Molinnus, Denise
AU - Drinic, Aleksander
AU - Iken, Heiko
AU - Kröger, Nadja
AU - Zinser, Max
AU - Smeets, Ralf
AU - Köpf, Marius
AU - Kopp, Alexander
AU - Schöning, Michael J
N1 - Copyright © 2021 Elsevier B.V. All rights reserved.
PY - 2021/7/1
Y1 - 2021/7/1
N2 - In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. The flexible glucose biosensor is encapsulated by a biocompatible silicon rubber which is certificated for a safe use onto human skin. Characterization of the sensor set-up is exemplarily demonstrated by glucose measurements in buffer and Ringer's solution, while the stability of the quasi-reference electrode has been investigated versus a commercial Ag/AgCl reference electrode. Repeated bending studies validated the mechanical properties of the electrode structures. The cross-sensitivity of the biosensor against ascorbic acid, noradrenaline and adrenaline was investigated, too. Additionally, biocompatibility and degradation tests of the silk fibroin with and without thin-film platinum electrodes were carried out.
AB - In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. The flexible glucose biosensor is encapsulated by a biocompatible silicon rubber which is certificated for a safe use onto human skin. Characterization of the sensor set-up is exemplarily demonstrated by glucose measurements in buffer and Ringer's solution, while the stability of the quasi-reference electrode has been investigated versus a commercial Ag/AgCl reference electrode. Repeated bending studies validated the mechanical properties of the electrode structures. The cross-sensitivity of the biosensor against ascorbic acid, noradrenaline and adrenaline was investigated, too. Additionally, biocompatibility and degradation tests of the silk fibroin with and without thin-film platinum electrodes were carried out.
KW - Animals
KW - Biocompatible Materials
KW - Biosensing Techniques
KW - Bombyx
KW - Fibroins
KW - Humans
KW - Polymers
KW - Silk
U2 - 10.1016/j.bios.2021.113204
DO - 10.1016/j.bios.2021.113204
M3 - SCORING: Journal article
C2 - 33836429
VL - 183
JO - BIOSENS BIOELECTRON
JF - BIOSENS BIOELECTRON
SN - 0956-5663
M1 - 113204
ER -