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The vesicular release probability sets the strength of individual Schaffer collateral synapses

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The vesicular release probability sets the strength of individual Schaffer collateral synapses. / Dürst, Céline; Wiegert, J. Simon; Schulze, Christian; Helassa, Nordine; Torok, Katalin; Oertner, Thomas.

bioRxiv : Cold Spring Harbor Laboratory Press. 2020, Preprint.

Research output: Other contribution › Other › Research

Harvard

Dürst, C, Wiegert, JS, Schulze, C, Helassa, N, Torok, K & Oertner, T 2020, The vesicular release probability sets the strength of individual Schaffer collateral synapses. Cold Spring Harbor Laboratory Press, bioRxiv. https://doi.org/10.1101/2020.08.02.232850

APA

Dürst, C., Wiegert, J. S., Schulze, C., Helassa, N., Torok, K., & Oertner, T. (2020, Oct 14). The vesicular release probability sets the strength of individual Schaffer collateral synapses. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/2020.08.02.232850

Vancouver

Dürst C, Wiegert JS, Schulze C, Helassa N, Torok K, Oertner T. The vesicular release probability sets the strength of individual Schaffer collateral synapses. 2020. https://doi.org/10.1101/2020.08.02.232850

Bibtex

@misc{3b7124add9aa4e0184e3a7aeeeefcf27,
title = "The vesicular release probability sets the strength of individual Schaffer collateral synapses",
abstract = "Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. Analyzing the variability of quantal parameters, we conclude that the vesicular release probability rather than the number of readily releasable vesicles or their transmitter content determines the potency of individual boutons.",
author = "C{\'e}line D{\"u}rst and Wiegert, {J. Simon} and Christian Schulze and Nordine Helassa and Katalin Torok and Thomas Oertner",
year = "2020",
month = oct,
day = "14",
doi = "10.1101/2020.08.02.232850",
language = "English",
publisher = "Cold Spring Harbor Laboratory Press",
address = "United States",
type = "Other",

}

RIS

TY - GEN

T1 - The vesicular release probability sets the strength of individual Schaffer collateral synapses

AU - Dürst, Céline

AU - Wiegert, J. Simon

AU - Schulze, Christian

AU - Helassa, Nordine

AU - Torok, Katalin

AU - Oertner, Thomas

PY - 2020/10/14

Y1 - 2020/10/14

N2 - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. Analyzing the variability of quantal parameters, we conclude that the vesicular release probability rather than the number of readily releasable vesicles or their transmitter content determines the potency of individual boutons.

AB - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. Analyzing the variability of quantal parameters, we conclude that the vesicular release probability rather than the number of readily releasable vesicles or their transmitter content determines the potency of individual boutons.

U2 - 10.1101/2020.08.02.232850

DO - 10.1101/2020.08.02.232850

M3 - Other

PB - Cold Spring Harbor Laboratory Press

CY - bioRxiv

ER -