The vesicular release probability sets the strength of individual Schaffer collateral synapses
Standard
The vesicular release probability sets the strength of individual Schaffer collateral synapses. / Dürst, Céline; Wiegert, J. Simon; Schulze, Christian; Helassa, Nordine; Torok, Katalin; Oertner, Thomas.
bioRxiv : Cold Spring Harbor Laboratory Press. 2020Preprint.Publikationen: Andere Beiträge › Andere › Forschung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - GEN
T1 - The vesicular release probability sets the strength of individual Schaffer collateral synapses
AU - Dürst, Céline
AU - Wiegert, J. Simon
AU - Schulze, Christian
AU - Helassa, Nordine
AU - Torok, Katalin
AU - Oertner, Thomas
PY - 2020/10/14
Y1 - 2020/10/14
N2 - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. Analyzing the variability of quantal parameters, we conclude that the vesicular release probability rather than the number of readily releasable vesicles or their transmitter content determines the potency of individual boutons.
AB - Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. Analyzing the variability of quantal parameters, we conclude that the vesicular release probability rather than the number of readily releasable vesicles or their transmitter content determines the potency of individual boutons.
U2 - 10.1101/2020.08.02.232850
DO - 10.1101/2020.08.02.232850
M3 - Other
PB - Cold Spring Harbor Laboratory Press
CY - bioRxiv
ER -