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The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study

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The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study. / Tabatabaei-Panah, Pardis-Sadat; Moravvej, Hamideh; Hajihasani, Mahsa; Mousavi, Mahsa; Ludwig, Ralf J; Akbarzadeh, Reza.

In: IMMUN INFLAMM DIS, Vol. 10, No. 2, 02.2022, p. 209-217.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Tabatabaei-Panah, P-S, Moravvej, H, Hajihasani, M, Mousavi, M, Ludwig, RJ & Akbarzadeh, R 2022, 'The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study', IMMUN INFLAMM DIS, vol. 10, no. 2, pp. 209-217. https://doi.org/10.1002/iid3.564

APA

Tabatabaei-Panah, P-S., Moravvej, H., Hajihasani, M., Mousavi, M., Ludwig, R. J., & Akbarzadeh, R. (2022). The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study. IMMUN INFLAMM DIS, 10(2), 209-217. https://doi.org/10.1002/iid3.564

Vancouver

Tabatabaei-Panah P-S, Moravvej H, Hajihasani M, Mousavi M, Ludwig RJ, Akbarzadeh R. The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study. IMMUN INFLAMM DIS. 2022 Feb;10(2):209-217. https://doi.org/10.1002/iid3.564

Bibtex

@article{95fb4bde01bf4ec3b4c433d32851a74f,
title = "The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study",
abstract = "BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in MCP-1 as well as in methylene-tetrahydrofolate reductase (MTHFR), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between MCP-1 and MTHFR variations and altered expression of their genes and the risk of AA.METHODS: Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of MCP-1 and MTHFR were evaluated by real-time reverse-transcription polymerase chain reaction analysis. Moreover, MCP-1 rs1024611 (A-2518G) and MTHFR rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction-restriction fragment length polymorphism assays.RESULTS: In contrast to MCP-1, the MTHFR gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in MCP-1 (AG + GG) and MTHFR (CT + TT) could significantly alter gene expression levels. Elevation of MCP-1 expression was significantly associated with the total number of variant MCP-1 and MTHFR alleles. However, no statistically significant difference was noticed in the genotypic distribution of MCP-1 and MTHFR variations between patients and controls.CONCLUSION: In summary, despite MCP-1 rs1024611 and MTHFR rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing MCP-1 activity.",
keywords = "Alopecia Areata/genetics, Chemokine CCL2/genetics, Genetic Predisposition to Disease, Humans, Methylenetetrahydrofolate Reductase (NADPH2)/genetics, Pilot Projects, Polymorphism, Single Nucleotide",
author = "Pardis-Sadat Tabatabaei-Panah and Hamideh Moravvej and Mahsa Hajihasani and Mahsa Mousavi and Ludwig, {Ralf J} and Reza Akbarzadeh",
note = "{\textcopyright} 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.",
year = "2022",
month = feb,
doi = "10.1002/iid3.564",
language = "English",
volume = "10",
pages = "209--217",
journal = "IMMUN INFLAMM DIS",
issn = "2050-4527",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study

AU - Tabatabaei-Panah, Pardis-Sadat

AU - Moravvej, Hamideh

AU - Hajihasani, Mahsa

AU - Mousavi, Mahsa

AU - Ludwig, Ralf J

AU - Akbarzadeh, Reza

N1 - © 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

PY - 2022/2

Y1 - 2022/2

N2 - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in MCP-1 as well as in methylene-tetrahydrofolate reductase (MTHFR), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between MCP-1 and MTHFR variations and altered expression of their genes and the risk of AA.METHODS: Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of MCP-1 and MTHFR were evaluated by real-time reverse-transcription polymerase chain reaction analysis. Moreover, MCP-1 rs1024611 (A-2518G) and MTHFR rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction-restriction fragment length polymorphism assays.RESULTS: In contrast to MCP-1, the MTHFR gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in MCP-1 (AG + GG) and MTHFR (CT + TT) could significantly alter gene expression levels. Elevation of MCP-1 expression was significantly associated with the total number of variant MCP-1 and MTHFR alleles. However, no statistically significant difference was noticed in the genotypic distribution of MCP-1 and MTHFR variations between patients and controls.CONCLUSION: In summary, despite MCP-1 rs1024611 and MTHFR rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing MCP-1 activity.

AB - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in MCP-1 as well as in methylene-tetrahydrofolate reductase (MTHFR), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between MCP-1 and MTHFR variations and altered expression of their genes and the risk of AA.METHODS: Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of MCP-1 and MTHFR were evaluated by real-time reverse-transcription polymerase chain reaction analysis. Moreover, MCP-1 rs1024611 (A-2518G) and MTHFR rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction-restriction fragment length polymorphism assays.RESULTS: In contrast to MCP-1, the MTHFR gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in MCP-1 (AG + GG) and MTHFR (CT + TT) could significantly alter gene expression levels. Elevation of MCP-1 expression was significantly associated with the total number of variant MCP-1 and MTHFR alleles. However, no statistically significant difference was noticed in the genotypic distribution of MCP-1 and MTHFR variations between patients and controls.CONCLUSION: In summary, despite MCP-1 rs1024611 and MTHFR rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing MCP-1 activity.

KW - Alopecia Areata/genetics

KW - Chemokine CCL2/genetics

KW - Genetic Predisposition to Disease

KW - Humans

KW - Methylenetetrahydrofolate Reductase (NADPH2)/genetics

KW - Pilot Projects

KW - Polymorphism, Single Nucleotide

U2 - 10.1002/iid3.564

DO - 10.1002/iid3.564

M3 - SCORING: Journal article

C2 - 34752683

VL - 10

SP - 209

EP - 217

JO - IMMUN INFLAMM DIS

JF - IMMUN INFLAMM DIS

SN - 2050-4527

IS - 2

ER -