The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study
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The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study. / Tabatabaei-Panah, Pardis-Sadat; Moravvej, Hamideh; Hajihasani, Mahsa; Mousavi, Mahsa; Ludwig, Ralf J; Akbarzadeh, Reza.
in: IMMUN INFLAMM DIS, Jahrgang 10, Nr. 2, 02.2022, S. 209-217.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The MCP-1 rs1024611 and MTHFR rs1801133 gene variations and expressions in alopecia areata: A pilot study
AU - Tabatabaei-Panah, Pardis-Sadat
AU - Moravvej, Hamideh
AU - Hajihasani, Mahsa
AU - Mousavi, Mahsa
AU - Ludwig, Ralf J
AU - Akbarzadeh, Reza
N1 - © 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.
PY - 2022/2
Y1 - 2022/2
N2 - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in MCP-1 as well as in methylene-tetrahydrofolate reductase (MTHFR), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between MCP-1 and MTHFR variations and altered expression of their genes and the risk of AA.METHODS: Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of MCP-1 and MTHFR were evaluated by real-time reverse-transcription polymerase chain reaction analysis. Moreover, MCP-1 rs1024611 (A-2518G) and MTHFR rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction-restriction fragment length polymorphism assays.RESULTS: In contrast to MCP-1, the MTHFR gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in MCP-1 (AG + GG) and MTHFR (CT + TT) could significantly alter gene expression levels. Elevation of MCP-1 expression was significantly associated with the total number of variant MCP-1 and MTHFR alleles. However, no statistically significant difference was noticed in the genotypic distribution of MCP-1 and MTHFR variations between patients and controls.CONCLUSION: In summary, despite MCP-1 rs1024611 and MTHFR rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing MCP-1 activity.
AB - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in MCP-1 as well as in methylene-tetrahydrofolate reductase (MTHFR), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between MCP-1 and MTHFR variations and altered expression of their genes and the risk of AA.METHODS: Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of MCP-1 and MTHFR were evaluated by real-time reverse-transcription polymerase chain reaction analysis. Moreover, MCP-1 rs1024611 (A-2518G) and MTHFR rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction-restriction fragment length polymorphism assays.RESULTS: In contrast to MCP-1, the MTHFR gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in MCP-1 (AG + GG) and MTHFR (CT + TT) could significantly alter gene expression levels. Elevation of MCP-1 expression was significantly associated with the total number of variant MCP-1 and MTHFR alleles. However, no statistically significant difference was noticed in the genotypic distribution of MCP-1 and MTHFR variations between patients and controls.CONCLUSION: In summary, despite MCP-1 rs1024611 and MTHFR rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing MCP-1 activity.
KW - Alopecia Areata/genetics
KW - Chemokine CCL2/genetics
KW - Genetic Predisposition to Disease
KW - Humans
KW - Methylenetetrahydrofolate Reductase (NADPH2)/genetics
KW - Pilot Projects
KW - Polymorphism, Single Nucleotide
U2 - 10.1002/iid3.564
DO - 10.1002/iid3.564
M3 - SCORING: Journal article
C2 - 34752683
VL - 10
SP - 209
EP - 217
JO - IMMUN INFLAMM DIS
JF - IMMUN INFLAMM DIS
SN - 2050-4527
IS - 2
ER -