The influence of dietary lectins on the cell proliferation of human breast cancer cell lines in vitro
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The influence of dietary lectins on the cell proliferation of human breast cancer cell lines in vitro. / Valentiner, Ursula; Fabian, Stefanie; Schumacher, Udo; Leathem, Anthony J.
In: ANTICANCER RES, Vol. 23, No. 2B, 25.06.2003, p. 1197-206.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The influence of dietary lectins on the cell proliferation of human breast cancer cell lines in vitro
AU - Valentiner, Ursula
AU - Fabian, Stefanie
AU - Schumacher, Udo
AU - Leathem, Anthony J
PY - 2003/6/25
Y1 - 2003/6/25
N2 - BACKGROUND: Lectins, carbohydrate proteins, bind non-covalently to glycoconjugate of normal and malignant cells. If used in cell culture, they can influence cellular proliferation. In this study the in vitro effects of six dietary lectins on the cell proliferation of human breast cancer cell lines were investigated.MATERIALS AND METHODS: Cell proliferation was assessed by a colorimetric XTT-based assay kit. Lectin binding was characterized by lectin histochemistry.RESULTS: WGA considerably influenced the cell growth of all tested cell lines (MCF-7, T 47D, HBL 100, BT 20), whereas the effects of PHA-L, SBA and HPA were smaller, began at higher concentrations and were restricted to three cell lines (MCF-7, T 47D and HBL 100 for PHA-L; MCF-7, T 47D and BT 20 for SBA, respectively) and to one cell line (HBL 100 for HPA). STA and PNA had no effect at all.CONCLUSION: The present data suggested that some dietary lectins can inhibit cell growth of human breast cancer cells in vitro. These findings would argue for a protective effect of these plant lectins for breast cancer.
AB - BACKGROUND: Lectins, carbohydrate proteins, bind non-covalently to glycoconjugate of normal and malignant cells. If used in cell culture, they can influence cellular proliferation. In this study the in vitro effects of six dietary lectins on the cell proliferation of human breast cancer cell lines were investigated.MATERIALS AND METHODS: Cell proliferation was assessed by a colorimetric XTT-based assay kit. Lectin binding was characterized by lectin histochemistry.RESULTS: WGA considerably influenced the cell growth of all tested cell lines (MCF-7, T 47D, HBL 100, BT 20), whereas the effects of PHA-L, SBA and HPA were smaller, began at higher concentrations and were restricted to three cell lines (MCF-7, T 47D and HBL 100 for PHA-L; MCF-7, T 47D and BT 20 for SBA, respectively) and to one cell line (HBL 100 for HPA). STA and PNA had no effect at all.CONCLUSION: The present data suggested that some dietary lectins can inhibit cell growth of human breast cancer cells in vitro. These findings would argue for a protective effect of these plant lectins for breast cancer.
KW - Adenocarcinoma
KW - Breast
KW - Breast Neoplasms
KW - Carbohydrate Metabolism
KW - Cell Division
KW - Cells, Cultured
KW - Diet
KW - Epithelial Cells
KW - Female
KW - Humans
KW - Lectins
KW - Peanut Agglutinin
KW - Phytohemagglutinins
KW - Plant Lectins
KW - Soybean Proteins
KW - Tissue Fixation
KW - Tumor Cells, Cultured
KW - Wheat Germ Agglutinins
M3 - SCORING: Journal article
C2 - 12820371
VL - 23
SP - 1197
EP - 1206
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 2B
ER -