The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
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The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. / Schulz, Eike C; Mehrabi, Pedram; Müller-Werkmeister, Henrike M; Tellkamp, Friedjof; Jha, Ajay; Stuart, William; Persch, Elke; De Gasparo, Raoul; Diederich, François; Pai, Emil F; Miller, R J Dwayne.
In: NAT METHODS, Vol. 15, No. 11, 11.2018, p. 901-904.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
AU - Schulz, Eike C
AU - Mehrabi, Pedram
AU - Müller-Werkmeister, Henrike M
AU - Tellkamp, Friedjof
AU - Jha, Ajay
AU - Stuart, William
AU - Persch, Elke
AU - De Gasparo, Raoul
AU - Diederich, François
AU - Pai, Emil F
AU - Miller, R J Dwayne
PY - 2018/11
Y1 - 2018/11
N2 - We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
AB - We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
KW - Crystallography, X-Ray
KW - Equipment Design
KW - Hydrolases/chemistry
KW - Models, Molecular
KW - Protein Conformation
KW - Rhodopseudomonas/enzymology
KW - Synchrotrons/instrumentation
KW - Time Factors
U2 - 10.1038/s41592-018-0180-2
DO - 10.1038/s41592-018-0180-2
M3 - SCORING: Journal article
C2 - 30377366
VL - 15
SP - 901
EP - 904
JO - NAT METHODS
JF - NAT METHODS
SN - 1548-7091
IS - 11
ER -